Our products are not in aseptic condition. We cannot guarantee that the products won‘t contaminate your cells. However, a few precautions tips are proposed to avoid:First, the media must contain ampicillin which can effectively avoid most bacterial contamination.In addition, dissolve our products with pure DMSO or ethanol.These two solvent can be sterilized.Finally, all the experiments should be kept in a sterile environment.
For the majority of compounds, they could be dissolved in DMSO or other organic solvent. Their stock solution can be stored at -20°C for up to 3 months and aliquoted for sampling convenience. Several freeze/thaw cycles should not damage the activity of our small molecule products. However, in many instances the aqueous solutions of some compounds need to be made fresh and cannot be stored. For these compounds, their solution should be prepared just before use and stored no longer than 24 hours.
It is common for compounds to precipitate when a DMSO stock solution is diluted with aqueous media. Most of these precipitates will re-dissolve with a few minutes of vortexing, sonication, or heating the solution in a 37℃ water bath with sonication. Please ensure that the precipitate has completely re-dissolved before using the solution.
Warming the solution up to 37℃ for brief periods combined with either vortexing or sonication for several minutes is often sufficient to dissolve the compound. If the solid does not dissolve after these steps, please contact our technical support for further assistance.
Please reconstitute peptides with sterile water. For acidic peptides or basic peptides, small amounts of ammonium hydroxide or 10% acetic acid should facilitate dissolution. For hydrophobic or neutral peptides, a minimal amount of DMSO or DMF should first be used to solubilize the peptide. Then, add water to buffer the solution. If necessary the mixture can be sonicated
The information about optional solvents for our products can be found in our product datasheet. For many compounds, they are not directly soluble in water, PBS or saline, but they can be dissolved in alcohol or DMSO to make a stock solution. These stock solutions are often stable for storage and can be diluted to the desired concentration with aqueous media for use in vitro and in vivo assays. In most cases, solubility in organic solvent will be high enough to warrant dilutions of 1/500 to 1/1000 with aqueous buffer according to your experimental objective. Small amounts of residual organic solvent (for example 0.1% DMSO) do not affect most of the biological assays, however we recommend solvent controls be run alongside all experiments.
If the quantity of the products you buy is very little and some products are adhered on pipe wall during the process of freeze-drying forming a thin layer, it may not be observed. You can add the specified solvent (refer to manual), vortex or ultrasonic oscillation until completely dissolved.