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5-BrdU

Catalog No. T6794   CAS 59-14-3
Synonyms: BUdR, Broxuridine, Bromodeoxyuridine, BRDU

5-BrdU (Broxuridine) , a nucleoside analog, are used in the detection of proliferating cells and competes with thymidine for incorporation into DNA.

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5-BrdU Chemical Structure
5-BrdU, CAS 59-14-3
Pack Size Availability Price/USD Quantity
50 mg In stock $ 30.00
100 mg In stock $ 42.00
200 mg In stock $ 47.00
500 mg In stock $ 58.00
1 g In stock $ 89.00
1 mL * 10 mM (in DMSO) In stock $ 46.00
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Purity: 100%
Purity: 99.87%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description 5-BrdU (Broxuridine) , a nucleoside analog, are used in the detection of proliferating cells and competes with thymidine for incorporation into DNA.
In vitro In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. [1] BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. [2]
In vivo In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. [1]
Kinase Assay Assays are run in the presence of 100 μM ATP using 10 μM of substrate. 30 μL PROTEIN-MIX in 25% DMSO and incubated for 15 min at room temperature. 10 μL PEPTIDE-MIX is added, the mixture is incubated for 60 min at RT and stopped by adding 180 μL 6.4% TCA (final concentration: 5%). Incorporated phosphate is measured in a scintillation counter and IC50 values are calculated using a sigmoidal curve analysis program with variable hill slope[1].
Cell Research Cultures are initially plated at 2000 cells/cm2 and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 μM BrdU for 24 hours, and cumulative growth curves were obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.(Only for Reference)
Synonyms BUdR, Broxuridine, Bromodeoxyuridine, BRDU
Molecular Weight 307.1
Formula C9H11BrN2O5
CAS No. 59-14-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 2 mg/mL(6.5 mM)

H2O: 15.4 mg/mL(50 mM)

DMSO: 61 mg/mL (198.63 mM)

TargetMolReferences and Literature

1. Levkoff LH, et al. Neoplasia. 2008, 10(8), 804-816. 2. Rothaeusler K, et al. Curr Protoc Cytom. 2007, Chapter 7, Unit7, 31.

TargetMolCitations

1. Wang W, Ren S, Lu Y, et al. Inhibition of Syk promotes chemical reprogramming of fibroblasts via metabolic rewiring and H2S production. The EMBO Journal. 2021 Jun 1;40(11):e106771. doi: 10.15252/embj.2020106771. Epub 2021 Apr 28. 2. Huang J, Fan H, Chen Y M, et al.The salt-inducible kinases inhibitor HG-9-91-01 exhibits antidepressant-like actions in mice exposed to chronic unpredictable mild stress.Neuropharmacology.2023: 109437.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library Anti-Cancer Active Compound Library Anti-Aging Compound Library Drug Repurposing Compound Library Anti-Cancer Compound Library Anti-Infection Compound Library Anti-Viral Compound Library Pediatric Drug Library NO PAINS Compound Library

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Keywords

5-BrdU 59-14-3 Cell Cycle/Checkpoint DNA Damage/DNA Repair Others Nucleoside Antimetabolite/Analog 5BrdU inhibit BUdR Broxuridine Inhibitor Bromodeoxyuridine 5 BrdU BRDU inhibitor

 

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