Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Amuvatinib (MP470) is an orally bioavailable synthetic carbothioamide with potential antineoplastic activity.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 44.00 | |
2 mg | In stock | $ 62.00 | |
5 mg | In stock | $ 93.00 | |
10 mg | In stock | $ 150.00 | |
25 mg | In stock | $ 275.00 | |
50 mg | In stock | $ 441.00 | |
100 mg | In stock | $ 556.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 101.00 |
Description | Amuvatinib (MP470) is an orally bioavailable synthetic carbothioamide with potential antineoplastic activity. |
Targets&IC50 | c-Kit (D816H):10 nM, PDGFRα (V561D):40 nM, FLT3 (D835Y):81 nM |
Kinase Assay | Kinase inhibition assay of c-Kit and PDGFRα: For the testing of inhibitory activity against c-Kit and PDGFRα, enzymes are incubated with varying concentrations of MP-470 and radiolabeled γ-32P-ATP. After 30 min, the reaction mixtures are electrophoresed on an acrylamide gel and autophosphorylation, quantitated by the amount of radioactivity incorporated into the enzyme, is assayed. |
Cell Research | Cells are plated at a density of 2 × 103 to 1 × 104 cells per well in 100 μL medium on day 0 in 96-well Falcon microtitier plates. On day 1, ten μL of serial dilutions of MP-470 are added to the plates in quadruplicates. After incubation for 4 days, the cells are fixed with 10% Trichloroacetic acid solution. Subsequently, they are labeled with 0.04% Sulforhodamine B (SRB) in 1% acetic acid. After multiple washes to remove the excess dye, 100 μL of 50 mM Tris solution is added to each well in order to dissolve the dye. The absorbance of each well is read on a plate reader at 570 nm. Date are expressed as the percentage of survival of control calculated from the absorbance corrected for background absorbance. The surviving percent of cells is determined by dividing the mean absorbance values of the monoclonal antibody by mean absorbance values of the control and multiplying by 100.(Only for Reference) |
Synonyms | MP470, HPK 56 |
Molecular Weight | 447.51 |
Formula | C23H21N5O3S |
CAS No. | 850879-09-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30 mg/mL (67 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Amuvatinib 850879-09-3 Angiogenesis Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair Tyrosine Kinase/Adaptors FLT c-Met/HGFR c-RET DNA/RNA Synthesis c-Kit PDGFR HPK56 MP 470 MP-470 HPK-56 Fms like tyrosine kinase 3 Cluster of differentiation antigen 135 RET FLT3 CD117 CD135 Platelet-derived growth factor receptor SCFR MP470 Inhibitor RAD51 HPK 56 inhibit inhibitor