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B-AP15

Catalog No. T1932 CAS 1009817-63-3

Synonyms: NSC 687852

B-AP15(NSC687852) is a selective inhibitor of the deubiquitinating enzymes Usp14 and UCHL5 of the 26S Proteasome. It blocks the deubiquitinating activity of the 26S Proteasome.

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B-AP15, CAS 1009817-63-3

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	B-AP15(NSC687852) is a selective inhibitor of the deubiquitinating enzymes Usp14 and UCHL5 of the 26S proteasome. It blocks the deubiquitinating activity of the 26S proteasome.
Targets&IC50	UCHL5:2.1 μM
Kinase Assay	For deubiquitinase inhibition assays, 19S regulatory particle (5 nM), 26S (5 nM) UCH-L1 (5 nM), UCH-L3 (0.3 nM), USP2CD (5 nM) USP7CD (5 nM) USP8CD (5 nM) or BAP1 (5 nM) is incubated with DMSO or b-AP15 and monitored the cleavage of ubiquitin-AMC (1,000 nM) using a Wallac VICTOR Multilabel counter or a Tecan Infinite M1000 equipped with 380 nm excitation and 460 nm emission filters[1].
Cell Research	b-AP15 is dissolved in DMSO and stored, and then diluted with appropriate medium before use[2]. Cell viability is monitored by either the fluorometric microculture cytotoxicity assay or the MTT assay. For the MTT assay, cells are seeded into 96-well flat-bottomed plates overnight and exposed to drugs, using DMSO as the control. At the end of incubations, 10 μl of a stock solution of 5 mg/mL MTT is added into each well, and the plates are incubated 4 hours at 37°C. Formazan crystals are dissolved with 100 μL 10% SDS/10 mM HCl solution overnight at 37°C. Absorbance is measured using an enzyme-linked immunosorbent assay (ELISA) plate reader at 590 nm[2].

Synonyms	NSC 687852
Molecular Weight	419.39
Formula	C22H17N3O6
CAS No.	1009817-63-3

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

DMSO: 41.9 mg/mL (100 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. D'Arcy P, et al. Nat Med. 2011, 17(12):1636-40. 2. D'Arcy P, et al. Int J Biochem Cell Biol. 2012, 44(11):1729-38. 3. Lopez-Castejon G, et al. J Biol Chem, 2013, 288(4), 2721-2733.

Citations

1. Wu W, Xu H, Liao C, et al. Blockade of USP14 potentiates type I interferon signaling and radiation-induced antitumor immunity via preventing IRF3 deubiquitination. Cellular Oncology. 2022: 1-15

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

B-AP15 1009817-63-3 Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair Ubiquitination DUB Deubiquitinase Inhibitor BAP15 NSC 687852 inhibit NSC687852 DUBs NSC-687852 B AP15 b-AP15 B-AP-15 inhibitor

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