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Membrane transporter/Ion channel Sodium Channel Benzamil (hydrochloride)

Benzamil (hydrochloride)

Catalog No. T5850   CAS 2898-76-2
Purity 98.00% Datasheet

Benzamil is an epithelial sodium channel (ENaC) blocker that inhibits sodium transport from (IC50 = 4 nM) and binds to (Kd = 5 nM) bovine kidney cortex membrane vesicles.

Benzamil (hydrochloride), CAS 2898-76-2
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25 mg Inquiry 140.00
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100 mg Inquiry 480.00
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Purity 98.00%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Benzamil is an epithelial sodium channel (ENaC) blocker that inhibits sodium transport from (IC50 = 4 nM) and binds to (Kd = 5 nM) bovine kidney cortex membrane vesicles.
Targets&IC50 ENaC,  
Animal Research
On the day of the experiment, rats were allocated into experimental and control groups for the tested drugs.Control groups were performed using intrathecal DMSO (n=5) or methanol (n=5) according to the solvent used for the tested drugs.All experiments were performed by investigators blinded to the treatment.To assess the effects of both drugs, increasing doses of amiloride (1, 3, 10 μg in 10 μL, n=5-7) or benzamil (3, 10, 30 μg in 10 μL, n=5-7) were investigated.The withdrawal threshold was measured prior to spinal nerve ligation and was regarded as pre-ligated threshold.The withdrawal threshold was measured immediately prior to intrathecal drug delivery and was regarded as a post-ligated baseline threshold.The withdrawal threshold was determined at 15, 30, 60, 90, 120, 150, and 180 min following intrathecal administration of the experimental drugs.For the purpose of examining the behavioral changes by amiloride and benzamil, the highest dose of each drug was administered intrathecally to 10 additional rats.Motor function was assessed by the righting reflex and placing-stepping reflex[1].
Appearance solid
Molecular Weight 356.21
Formula C13H15Cl2N7O
CAS No. 2898-76-2

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Jeong S , Lee S H , Kim Y O , et al. Antinociceptive Effects of Amiloride and Benzamil in Neuropathic Pain Model Rats[J]. Journal of Korean Medical Science, 2013, 28(8). 2. Nishimura M , Ohtsuka K , Nanbu A , et al. Benzamil blockade of brain Na+ channels averts Na(+)-induced hypertension in rats.[J]. American Journal of Physiology, 1998, 274(2):635-44.

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