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CDDO-Im

Catalog No. TQ0120 CAS 443104-02-7

Synonyms: RTA-403, CDDO-Imidazolide, TP-235

CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

CDDO-Im, CAS 443104-02-7

Pack Size	Availability	Price/USD
1 mg	In stock	$ 31.00
5 mg	In stock	$ 72.00
10 mg	In stock	$ 113.00
25 mg	In stock	$ 222.00
50 mg	In stock	$ 355.00
100 mg	In stock	$ 538.00
500 mg	In stock	$ 1,150.00
1 mL * 10 mM (in DMSO)	In stock	$ 86.00

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Purity: 100%

Purity: 98.3%

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Description	CDDO-Im (TP-235) is an activator of Nrf2 and PPAR (Kis: 232/344 nM for PPARα/PPARγ).
Targets&IC50	PPARγ:344 nM (Ki), PPARα:232 nM (Ki)
In vitro	CDDO-Im is highly active in suppressing cellular proliferation of human leukemia and breast cancer cell lines (IC50 approximately 10-30 nM). In U937 leukemia cells, CDDO-Im also induces monocytic differentiation as measured by increased cell surface expression of CD11b and CD36 [1]. CDDO-Im treatment markedly induces cell cycle arrest at G2/M-phase and apoptosis in the triple-negative breast cancer cell lines, SUM159 and MDA-MB-231. The CD24?/EpCAM+ cells in SUM159 tumorspheres are significantly inhibited by CDDO-Im treatment. CDDO-Im also significantly decreases sphere-forming efficiency and tumorsphere size in both primary and secondary sphere cultures [2]. Treatment with CDDO-Im elevates protein levels of Nrf2, a transcription factor previously shown to bind ARE sequences and increases expression of a number of antioxidant and detoxification genes regulated by Nrf2 [3].
In vivo	CDDO-Im exhibits strong inhibition of de novo inducible nitric oxide synthase (iNOS) expression in primary mouse macrophages. Additionally, it effectively suppresses the proliferation of B16 murine melanoma and L1210 murine leukemia cells in vivo. Administering 10 nM (5.4 μg) of CDDO-Im significantly impedes the induction of iNOS by IFN-γ, while a dose as low as 1 nmol (0.54 μg) of CDDO-Im shows partial effectiveness [1].
Cell Research	CDDO-Im is dissolved in DMSO. SUM159 and MDA-MB-231 cells are seeded into each well of 96-well plates (1,000 cell/well) and treated the next day with vehicle control or CDDO-Im (1, 10, 50, 100 and 200 nM) for given incubation time. The absorbance is measured with a spectrophotometer to determine the cell proliferation rate [2].
Animal Research	Mice are injected i.p. with thioglycollate, and the resulting resident peritoneal macrophages are activated 3 days later with an i.p. injection of IFN-γ. CDDO and CDDO-Im are injected i.p. 30 min after IFN-γ. Macrophages are harvested 10 h later, cultured for 12 h, and then assayed for expression of iNOS protein and production of nitric oxide (NO) [1].

Synonyms	RTA-403, CDDO-Imidazolide, TP-235
Molecular Weight	541.72
Formula	C34H43N3O3
CAS No.	443104-02-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (92.30 mM), Sonication is recommended.

H2O: Insoluble

References and Literature

1. Place AE, et al. The novel synthetic triterpenoid, CDDO-imidazolide, inhibits inflammatory response and tumor growth in vivo. Clin Cancer Res. 2003 Jul;9(7):2798-806. 2. So JY, et al. A synthetic triterpenoid CDDO-Im inhibits tumorsphere formation by regulating stem cell signaling pathways in triple-negative breast cancer. PLoS One. 2014 Sep 17;9(9):e107616. 3. Liby K, et al. The synthetic triterpenoids, CDDO and CDDO-imidazolide, are potent inducers of heme oxygenase-1 and Nrf2/ARE signaling. Cancer Res. 2005 Jun 1;65(11):4789-98.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Anti-Obesity Compound Library Bioactive Compound Library DNA Damage & Repair Compound Library Lipid Metabolism Compound Library Mitochondria-Targeted Compound Library Anti-Diabetic Compound Library Anti-Breast Cancer Compound Library Ferroptosis Compound Library Cell Cycle Compound Library

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Keywords

CDDO-Im 443104-02-7 Apoptosis DNA Damage/DNA Repair Immunology/Inflammation Metabolism Others Ferroptosis Nrf2 PPAR inhibit Keap1-Nrf2 RTA 403 CDDO Im Inhibitor TP 235 Peroxisome proliferator-activated receptors RTA-403 RTA403 CDDOIm TP235 CDDO-Imidazolide TP-235 inhibitor

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