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CHIR-98014

Catalog No. T2608   CAS 252935-94-7
Synonyms: CHIR 98014, CT98014, CHIR98014

CHIR-98014 (CT98014) is a selective GSK3 inhibitor; potentiate insulin activation of glucose transport and utilization in vitro and in vivo.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
CHIR-98014 Chemical Structure
CHIR-98014, CAS 252935-94-7
Pack Size Availability Price/USD Quantity
5 mg In stock $ 68.00
10 mg In stock $ 109.00
25 mg In stock $ 228.00
50 mg In stock $ 372.00
100 mg In stock $ 597.00
500 mg In stock $ 1,260.00
1 mL * 10 mM (in DMSO) In stock $ 73.00
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Purity: 99.41%
Purity: 97.86%
Purity: 97.09%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description CHIR-98014 (CT98014) is a selective GSK3 inhibitor; potentiate insulin activation of glucose transport and utilization in vitro and in vivo.
Targets&IC50 GSK-3α:0.65 nM, GSK-3β:0.58 nM
Kinase Assay Kinase assays: Polypropylene 96-well plates are ?lled with 300 μL/well buffer (50 mM tris HCl, 10 mM MgCl2, 1 mM EGTA, 1 mM dithiothreitol, 25 mM β-glycerophosphate, 1 mM NaF, 0.01% BSA, pH 7.5) containing kinase, peptide substrate, and any activators. CHIR-98014 or controls are added in 3.5 μL of DMSO, followed by 50 μL of ATP stock to yield a ?nal concentration of 1 μM ATP in all cell-free assays. After incubation, triplicate 100-μL aliquots are transferred to Combiplate eight plates containing 100 μL/well 50 μM ATP and 20 mM EDTA. After 1 hour, the wells are rinsed ?ve times with PBS, ?lled with 200 μL of scintillation ?uid, sealed, left 30 min, and counted in a scintillation counter. All steps are performed at room temperature.
Cell Research CHO-IR cells expressing human insulin receptor are grown to 80% con?uence in Hamm’s F12 medium with 10% fetal bovine serum and without hypoxanthine. Trypsinized cells are seeded in 6-well plates at 1 × 106 cells/well in 2 mL of medium without fetal bovine serum. After 24 hours, medium is replaced with 1 mL of serum-free medium containing GSK-3 inhibitor CHIR 98014 or control (?nal DMSO concentration 0.1%) for 30 min at 37 °C. Cells are lysed by freeze/thaw in 50 mM tris (pH 7.8) containing 1 mM EDTA, 1 mM DTT, 100 mM NaF, 1 mM phenylmethylsulfonyl ?uoride, and 25 μg/mL leupeptin (buffer A) and centrifuged 15 min at 4 °C/14000 g. The activity ratio of GS is calculated as the GS activity in the absence of glucose-6-phosphate divided by the activity in the presence of 5 mM glucose-6-phosphate. (Only for Reference)
Synonyms CHIR 98014, CT98014, CHIR98014
Molecular Weight 486.31
Formula C20H17Cl2N9O2
CAS No. 252935-94-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 8 mg/mL (16.45 mM), warmed

TargetMolReferences and Literature

1. Ring DB,et al. Diabetes. 2003, 52(3), 588-595. 2. Selenica ML, et al. Br J Pharmacol. 2007, 152(6), 959-979.

Related compound libraries

This product is contained In the following compound libraries:
Tyrosine Kinase Inhibitor Library Anti-Neurodegenerative Disease Compound Library Highly Selective Inhibitor Library Inhibitor Library Cytokine Inhibitor Library Bioactive Compound Library Target-Focused Phenotypic Screening Library Anti-Cancer Metabolism Compound Library NO PAINS Compound Library Anti-Fibrosis Compound Library

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Keywords

CHIR-98014 252935-94-7 Angiogenesis MAPK PI3K/Akt/mTOR signaling Stem Cells Tyrosine Kinase/Adaptors FGFR GSK-3 S6 Kinase Src Glycogen synthase kinase-3 CT-98014 CHIR 98014 Inhibitor CT98014 CHIR98014 inhibit CT 98014 Glycogen synthase kinase 3 inhibitor

 

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