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DAPI Dihydrochloride

Catalog No. T19827 CAS 28718-90-3

Synonyms: FxCycle Violet, 4',6-diamidino-2-phenylindole

DAPI Dihydrochloride (FxCycle Violet) is a cell-permeable fluorescent probe by binding in the minor grove of A-T rich sequences of DNA, used to stain DNA and chromosomes, with a preference for adenine and thymine rich DNA.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

DAPI Dihydrochloride, CAS 28718-90-3

Pack Size	Availability	Price/USD
5 mg	In stock	$ 35.00
10 mg	In stock	$ 62.00
25 mg	In stock	$ 133.00
50 mg	In stock	$ 213.00
100 mg	In stock	$ 318.00
200 mg	In stock	$ 473.00
1 mL * 10 mM (in DMSO)	In stock	$ 39.00

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Purity: 99.94%

Purity: 99.81%

Biological Description

Chemical Properties

Storage & Solubility Information

Description

Synonyms	FxCycle Violet, 4',6-diamidino-2-phenylindole
Molecular Weight	350.24
Formula	C16H17Cl2N5
CAS No.	28718-90-3

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 8.33 mg/mL (23.78 mM), Sonification is recommended.

DMSO: 75 mg/mL (214.13 mM), Sonification is recommended.

References and Literature

1. Kapuscinski J, et al. DAPI: a DNA-specific fluorescent probe. Biotech Histochem. 1995 Sep;70(5):220-33. 2. Ci L, Huang Z, Liu Y, Liu Z, Wei G, Lu W. Amino-functionalized poloxamer 407 with both mucoadhesive and thermosensitive properties: preparation, characterization and application in a vaginal drug delivery system. Acta Pharm Sin B. 2017 Sep;7(5):593-602. doi: 10.1016/j.apsb.2017.03.002. Epub 2017 May 3. PubMed PMID: 28924553; PubMed Central PMCID: PMC5595263. 3. de Oliveira Souza A, Couto-Lima CA, Rosa Machado MC, Espreafico EM, Pinheiro Ramos RG, Alberici LC. Protective action of Omega-3 on paraquat intoxication in Drosophila melanogaster. J Toxicol Environ Health A. 2017;80(19-21):1050-1063. doi: 10.1080/15287394.2017.1357345. Epub 2017 Aug 29. PubMed PMID: 28849990. 4. Folarin OR, Snyder AM, Peters DG, Olopade F, Connor JR, Olopade JO. Brain Metal Distribution and Neuro-Inflammatory Profiles after Chronic Vanadium Administration and Withdrawal in Mice. Front Neuroanat. 2017 Jul 25;11:58. doi: 10.3389/fnana.2017.00058. eCollection 2017. PubMed PMID: 28790895; PubMed Central PMCID: PMC5524677. 5. Kobayashi D, Shibata A, Oike T, Nakano T. One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy. J Vis Exp. 2017 Oct 23;(128). doi: 10.3791/56338. PubMed PMID: 29155723.

Related compound libraries

This product is contained In the following compound libraries：

Human Metabolite Library

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

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Step Two: Enter the in vivo formulation

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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Molecular Weight Calculator

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Mass of a compound required to prepare a solution of known volume and concentration
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An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
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The answer of 19.713 mg appears in the Mass box

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Calculator the dilution required to prepare a stock solution

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The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
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The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

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Molecule Formula

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Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

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Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

DAPI Dihydrochloride 28718-90-3 Others inhibit FxCycle Violet Inhibitor DAPI 4',6-diamidino-2-phenylindole inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.