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Dehydrotrametenolic acid

Catalog No. TN1086 CAS 6879-05-6

Synonyms: Dehydroeburicoic acid

Dehydrotrametenolic acid (Dehydroeburicoic acid) induces necrotic cell death that involves Ca(2+) overload, mitochondrial dysfunction, and calpain activation in human glioblastomas.

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Dehydrotrametenolic acid Chemical Structure

Dehydrotrametenolic acid, CAS 6879-05-6

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Description	Dehydrotrametenolic acid (Dehydroeburicoic acid) induces necrotic cell death that involves Ca(2+) overload, mitochondrial dysfunction, and calpain activation in human glioblastomas.
In vitro	3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase release assays indicated that Dehydroeburicoic acid inhibited the proliferation of the human glioblastoma cell U87MG. In addition, Annexin V and propidium iodide staining showed that Dehydroeburicoic acid treatment led to a rapid increase of glioblastomas in the necrotic/late apoptotic fraction, whereas cell cycle analysis revealed that Dehydroeburicoic acid failed to significantly enhance the population of U87MG cells in the hypodiploid (sub-G1) fraction. Using electron microscopy, we found that Dehydroeburicoic acid induced significant cell enlargements, massive cytoplasmic vacuolization, and loss of mitochondrial membrane integrity. Dehydroeburicoic acid treatment triggered an intracellular Ca(2+) increase, and Dehydroeburicoic acid-induced cell death was significantly attenuated by BAPTA-AM but not ethylenediaminetetraacetic acid or ethylene glycol tetraacetic acid. Dehydroeburicoic acid instigated a reduction of both mitochondrial transmembrane potential and intracellular ATP level. Moreover, Dehydroeburicoic acid induced proteolysis of alpha-spectrin by calpain, and Dehydroeburicoic acid cytotoxicity in U87MG cells was caspase-independent but was effectively blocked by calpain inhibitor. Interestingly, Dehydroeburicoic acid also caused autophagic response that was prevented by calpain inhibitor.
Source	Natural Products > Polyporaceae > Wolfiporia

Synonyms	Dehydroeburicoic acid
Molecular Weight	468.71
Formula	C31H48O3
CAS No.	6879-05-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

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DMSO: 10 mM

References and Literature

1. Dehydroeburicoic acid induces calcium- and calpain-dependent necrosis in human U87MG glioblastomas.Chem Res Toxicol. 2009 Nov;22(11):1817-26.

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Keywords

Dehydrotrametenolic acid 6879-05-6 Apoptosis Immunology/Inflammation Proteases/Proteasome TNF NOS Caspase NO Synthase Dehydroeburicoic acid Inhibitor inhibit inhibitor

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