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Deoxycholic acid sodium salt

Catalog No. T5077 CAS 302-95-4

Synonyms: Sodium deoxycholate, Sodium Desoxycholate

Deoxycholic acid sodium salt (Sodium deoxycholate) can activate the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.

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Deoxycholic acid sodium salt Chemical Structure

Deoxycholic acid sodium salt, CAS 302-95-4

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50 mg	In stock	$ 38.00
100 mg	In stock	$ 54.00
500 mg	In stock	$ 76.00

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Purity: 99.77%

Purity: 99.75%

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Description	Deoxycholic acid sodium salt (Sodium deoxycholate) can activate the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.
In vitro	Deoxycholic acid (DCA) and chenoDeoxycholic acid (CDCA) are the common ingredients of duodenal reflux that act synergistically in many physiological and pathological processes. The cells are repeatedly exposed to 100 μM CDCA and Deoxycholic acid at pH 5.5 for up to 120 min. To simulate chronic local recurrent disease in vitro, the gastric cancer cell line MGC803 is exposed to acidified medium (pH 5.5) containing 100 μM Deoxycholic acid and CDCA. An untreated log-growth MGC803 cell line is generated to be used as a control in normal pH media. After daily 10 min exposure to the acidified bile acids for 60 weeks, MGC803-resistant cells are able to survive and proliferate after 120 min exposure [2].
Cell Research	MGC803 cells are cultured in Roswell Park Memorial Institute media supplemented with 10% fetal calf serum and 100 U/mL Penicillin and 100 mg/mL Streptomycin. To generate MGC803-resistant cells, the pH value of the MGC803 culture medium is adjusted to the experimental conditions using the hydrochloric acid (A). The bile acids GCDA and Deoxycholic acid are diluted to optimal working concentrations of 100 μM (B) with culture medium, and the overall pH (A+B) is adjusted to pH 5.5, simulating the gastric environment. Initially, MGC803 cells are chronically exposed to acidified medium with bile acids (A+B) for 10 min every 24 h. The experimental time and conditions are optimized in our preliminary experiments, which show that 10 min is enough and does not result in cell damage. This procedure is repeated and it takes 60 weeks for the MGC803 cells to survive and proliferate under the exposure of A+B for 120 min. Control untreated cells are cultured in neutral RPMI medium at pH 7.4 in parallel to the resistant cells for 60 weeks. The morphological changes in MGC803 cells exposed to acidified bile acids (A+B) are documented at 30 and 60 weeks[2].

Synonyms	Sodium deoxycholate, Sodium Desoxycholate
Molecular Weight	414.56
Formula	C24H39NaO4
CAS No.	302-95-4

Storage

keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 330 mg/mL

DMSO: 12.5 mg/mL (30.15 mM)

References and Literature

1. Somm E, et al. β-Klotho deficiency protects against obesity through a crosstalk between liver, microbiota, and brown adipose tissue. JCI Insight. 2017 Apr 20;2(8). pii: 91809. 2. Wang X, et al. Acidified bile acids enhance tumor progression and telomerase activity of gastric cancer in micedependent on c-Myc expression. Cancer Med. 2017 Apr;6(4):788-797.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Microbial Natural Product Library Drug Repurposing Compound Library GPCR Compound Library Anti-Cancer Approved Drug Library Membrane Protein-targeted Compound Library Anti-Cancer Drug Library Anti-Cancer Active Compound Library FDA-Approved & Pharmacopeia Drug Library Anti-Cancer Compound Library

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Keywords

Deoxycholic acid sodium salt 302-95-4 GPCR/G Protein Metabolism GPCR19 Endogenous Metabolite Inhibitor G-protein coupled receptor 19 bile acid TGR5 Deoxycholic acid G protein-coupled bile acid receptor GPBAR1 Deoxycholic acid sodium G protein-coupled Bile Acid Receptor 1 inhibit Sodium deoxycholate Sodium Desoxycholate inhibitor

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