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Ergolide

Catalog No. T13686 CAS 54999-07-4

Ergolide, a sesquiterpene lactone extracted from the dried flowers of Inula Britannica, effectively hinders the expression of inducible nitric oxide synthase and cyclo-oxygenase-2 in RAW 264.7 macrophages by deactivating NF-κB.

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Ergolide, CAS 54999-07-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 117.00
5 mg	In stock	$ 249.00
10 mg	In stock	$ 425.00
25 mg	In stock	$ 693.00
50 mg	In stock	$ 962.00
100 mg	In stock	$ 1,290.00
1 mL * 10 mM (in DMSO)	In stock	$ 297.00

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Purity: 99.56%

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Description

In vitro

Ergolide markedly decreased the production of prostaglandin E(2) (PGE(2)) in cell-free extract of LPS/IFN-gamma-stimulated RAW 264.7 macrophages in a concentration-dependent manner, without alteration of the catalytic activity of COX-2 itself.?Ergolide decreased the level of iNOS and COX-2 protein, and iNOS mRNA caused by stimulation of LPS/IFN-gamma in a concentration-dependent manner, as measured by Western blot and Northern blot analysis, respectively.?Ergolide inhibited nuclear factor-kappaB (NF-kappaB) activation, a transcription factor necessary for iNOS and COX-2 expression in response to LPS/IFN-gamma.?This effect was accompanied by the parallel reduction of nuclear translocation of subunit p65 of NF-kappaB as well as IkappaB-alpha degradation.?In addition, these effects were completely blocked by treatment of cysteine, indicating that this inhibitory effect of ergolide could be mediated by alkylation of NF-kappaB itself or an upstream molecule of NF-kappaB.?Ergolide also directly inhibited the DNA-binding activity of active NF-kappaB in LPS/IFN-gamma-pretreated RAW 264.7 macrophages.?These results demonstrate that the suppression of NF-kappaB activation by ergolide might be attributed to the inhibition of nuclear translocation of NF-kappaB resulted from blockade of the degradation of IkappaB and the direct modification of active NF-kappaB, leading to the suppression of the expression of iNOS and COX-2,?which play important roles in inflammatory signalling pathway.

Molecular Weight	306.35
Formula	C17H22O5
CAS No.	54999-07-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 50 mg/mL (163.21 mM), Sonification is recommended.

References and Literature

1. Whan Han J, et al. Ergolide, sesquiterpene lactone from Inula britannica, inhibits inducible nitric oxide synthase and cyclo-oxygenase-2 expression in RAW 264.7 macrophages through the inactivation of NF-kappaB. Br J Pharmacol. 2001 Jun;133(4):503-12.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Prostate Cancer Compound Library Antioxidant Compound Library Selected Plant-Sourced Compound Library Anti-Pancreatic Cancer Compound Library Bioactive Lipid Compound Library NF-κB Signaling Compound Library Covalent Natural Product Library Anti-Colorectal Cancer Traditional Chinese Medicine Compound Library Anti-Breast Cancer Compound Library

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Keywords

Ergolide 54999-07-4 NF-Κb NF-κB Nuclear factor-kappaB Nuclear factor-κB Inhibitor inhibit inhibitor

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