Home Tools

Ethyl 3,4-dihydroxybenzoate

Catalog No. T5684 CAS 3943-89-3

Ethyl 3,4-dihydroxybenzoate (EDHB): a prolyl hydroxylase inhibitor attenuates acute hypobaric hypoxia mediated vascular leakage in brain.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Ethyl 3,4-dihydroxybenzoate Chemical Structure

Ethyl 3,4-dihydroxybenzoate, CAS 3943-89-3

Pack Size	Availability	Price/USD	Quantity
500 mg	In stock	$ 41.00
1 mL * 10 mM (in DMSO)	In stock	$ 45.00

Bulk Inquiry

Select Batch

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Ethyl 3,4-dihydroxybenzoate (EDHB): a prolyl hydroxylase inhibitor attenuates acute hypobaric hypoxia mediated vascular leakage in brain.
In vitro	It is shown that DHB reversibly inhibits both morphological and biochemical differentiation of myoblasts, if it is added to the culture medium before the cell alignment stage.Along with the inhibition of procollagen synthesis, DHB also decreases markedly the production of a collagen-binding glycoprotein (gp46) present in the ER[1].
In vivo	EDHB supplementation effectively scaled down HH induced cerebral edema with concomitant downregulation of brain NF-κB expression.?There was significant curtailment of pro-inflammatory cytokines and cell adhesion molecules.?There was significant downregulation of permeability factor VEGF by EDHB with concomitant increment in hypoxia inducible factor (HIF1α) and anti-inflammatory proteins HO-1 and MT-1 compared to HH control thus accentuating the potential of EDHB as effective hypoxic preconditioning agent in ameliorating HH mediated injury in brain[2].
Animal Research	Male Sprague-Dawley rats treated with EDHB (75 mg/kg for 3 days), were subjected to acute HH exposure at 9144 m (30,000 ft) for 5 h. Animals were assessed for transvascular leakage and edema formation in brain and role of key inflammatory markers along with hypoxia responsive genes.

Molecular Weight	182.17
Formula	C9H10O4
CAS No.	3943-89-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 22.5 mg/mL (123.51 mM)

References and Literature

1. Nandan D , Clarke E P , Ball E H , et al. Ethyl-3,4-dihydroxybenzoate inhibits myoblast differentiation: Evidence for an essential role of collagen[J]. The Journal of Cell Biology, 1990, 110(5):1673-1679. 2. Singh D P , Nimker C , Paliwal P , et al. Ethyl 3,4-dihydroxybenzoate (EDHB): a prolyl hydroxylase inhibitor attenuates acute hypobaric hypoxia mediated vascular leakage in brain[J]. The Journal of Physiological Sciences, 2016, 66(4):315-326.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compound Library Anti-Inflammatory Traditional Chinese Medicine Compound Library NF-κB Signaling Compound Library Transcription Factor-Targeted Compound Library Anti-Tumor Natural Product Library Natural Product Library Anti-Breast Cancer Compound Library Anti-Obesity Compound Library Anti-Cancer Compound Library

Related Products

Related compounds with same targets

Benralizumab Decursin CPT2 OsMundacetone Acacetin COG-1410 acetate ICG-001 PP5-IN-1

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Ethyl 3,4-dihydroxybenzoate 3943-89-3 Apoptosis Autophagy Chromatin/Epigenetic Immunology/Inflammation Metabolism NF-Κb Others Reactive Oxygen Species NO Synthase HIF/HIF Prolyl-Hydroxylase antioxidant ROS NOS Ethyl 3,4 dihydroxybenzoate Hypoxia-inducible factors prolyl-hydroxylase inhibit HIF-PH Ethyl protocatechuate HIFs Ethyl 3,4dihydroxybenzoate Inhibitor collagen Nitric oxide synthases mitochondrial inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.