Home Tools

Fasudil hydrochloride

Catalog No. T3060 CAS 105628-07-7

Synonyms: AT877, Fasudil hydrochloride, TWNT-4 cells, TIMP-1, AT-877, a-SMA, Protein kinase A, Ca channels, PKA, HIV, rat HSCs, HA 1077, Rho-associated protein kinase, human HSC, ERK1/2, Rho-kinase, inhibit, PKC, Ca2+ channels, HA1077, Rho-associated kinase, HA-1077 hydrochloride, Fasudil HCl, p38, lysophoaphatidic acid, Fasudil, Ca2+ channel antagonist, JNK, Calcium Channel, protein kinases, collagen, LPA, ROCK, Fasudil (HA-1077) HCl, Inhibitor, Protein kinase C, vasodilator, ROK, Human immunodeficiency virus, Autophagy, orally active, HA-1077, AT 877

Fasudil Hydrochloride is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Fasudil hydrochloride, CAS 105628-07-7

Please contact us for prices and availability for the specification of product you are interested at.
Product consultation

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Fasudil Hydrochloride is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.
Targets&IC50	ROCK1:0.33 μM(ki), PKA:1.0 μM(ki), PKC:9.3 μM(ki), MLCK:55 μM(ki)
Kinase Assay	Cyclic AMP-dependent protein kinase activity is assayed in a reaction mixture containing, in a final volume of 0.2 mL, 50 mM Tris-HCl (pH 7.0), 10 mM magnesium acetate, 2 mM EGTA, 1 μM cyclic AMP or absence of cyclic AMP, 3.3 to 20 μM [r-32P] ATP (4×105 c.p.m.), 0.5 μg of the enzyme, 100 μg of histone H2B and compound. The mixture is incubated at 30°C for 5 min. The reaction is terminated by adding 1mL of ice-cold 20% trichloroacetic acid after adding 500 μg of bovine serum albumin as a carrier protein. The sample is centrifuged at 3000 r.p.m. for 15min, the pellet is resuspended in ice-cold 10% trichloro-acetic acid solution and the centrifugation-resuspension cycle is repeated three times. The final pellet is dissolved in 1 mL of 1 N NaOH and radioactivity is measured with a liquid scintillation counter.

Synonyms	AT877, Fasudil hydrochloride, TWNT-4 cells, TIMP-1, AT-877, a-SMA, Protein kinase A, Ca channels, PKA, HIV, rat HSCs, HA 1077, Rho-associated protein kinase, human HSC, ERK1/2, Rho-kinase, inhibit, PKC, Ca2+ channels, HA1077, Rho-associated kinase, HA-1077 hydrochloride, Fasudil HCl, p38, lysophoaphatidic acid, Fasudil, Ca2+ channel antagonist, JNK, Calcium Channel, protein kinases, collagen, LPA, ROCK, Fasudil (HA-1077) HCl, Inhibitor, Protein kinase C, vasodilator, ROK, Human immunodeficiency virus, Autophagy, orally active, HA-1077, AT 877
Molecular Weight	327.83
Formula	C14H18ClN3O2S
CAS No.	105628-07-7

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: 32.8 mg/mL (100 mM)

DMSO: 24.6 mg/mL (75 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature

1. Ono-Saito N, et al. Pharmacol Ther, 1999, 82(2-3), 123-131. 1. Sun L, Sun L, Li X, et al. A Novel Tigecycline Adjuvant ML-7 Reverses the Susceptibility of Tigecycline-Resistant Klebsiella pneumoniae. Frontiers in cellular and infection microbiology. 2022: 1341. 2. Asano T, et al. J Pharmacol Exp Ther, 1987, 241(3), 1033-1040. 3. Asano T, et al. Br J Pharmacol, 1989, 98(4), 1091-1100. 4. Negoro N, et al. Biochem Biophys Res Commun, 1999, 262(1), 211-215. 5. Fukushima M, et al. Liver Int, 2005, 25(4), 829-838.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Autophagy Compound Library GPCR Compound Library Anti-Cardiovascular Disease Compound Library TGF-beta/Smad Compound Library Inhibitor Library Ion Channel Inhibitor Library DNA Damage & Repair Compound Library Neural Regeneration Compound Library Anti-Metabolism Disease Compound Library

Related Products

Related compounds with same targets

Salinomycin sodium salt Physalin A Latifolin Lup-20(29)-en-28-oic acid R1487 Hydrochloride PTC-209 hydrobromide LYN-1604 ALLO-1

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.