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Fasudil hydrochloride

Catalog No. T3060   CAS 105628-07-7
Synonyms: AT877, Fasudil hydrochloride, TWNT-4 cells, TIMP-1, AT-877, a-SMA, Protein kinase A, Ca channels, PKA, HIV, rat HSCs, HA 1077, Rho-associated protein kinase, human HSC, ERK1/2, Rho-kinase, inhibit, PKC, Ca2+ channels, HA1077, Rho-associated kinase, HA-1077 hydrochloride, Fasudil HCl, p38, lysophoaphatidic acid, Fasudil, Ca2+ channel antagonist, JNK, Calcium Channel, protein kinases, collagen, LPA, ROCK, Fasudil (HA-1077) HCl, Inhibitor, Protein kinase C, vasodilator, ROK, Human immunodeficiency virus, Autophagy, orally active, HA-1077, AT 877

Fasudil Hydrochloride is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.

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Fasudil hydrochloride, CAS 105628-07-7
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Purity: 98%
Biological Description
Chemical Properties
Storage & Solubility Information
Description Fasudil Hydrochloride is a potent inhibitor of ROCK1, PKA, PKC, and MLCK with Ki of 0.33 μM, 1.0 μM, 9.3 μM and 55 μM, respectively.
Targets&IC50 ROCK1:0.33 μM(ki), PKA:1.0 μM(ki), PKC:9.3 μM(ki), MLCK:55 μM(ki)
Kinase Assay Cyclic AMP-dependent protein kinase activity is assayed in a reaction mixture containing, in a final volume of 0.2 mL, 50 mM Tris-HCl (pH 7.0), 10 mM magnesium acetate, 2 mM EGTA, 1 μM cyclic AMP or absence of cyclic AMP, 3.3 to 20 μM [r-32P] ATP (4×105 c.p.m.), 0.5 μg of the enzyme, 100 μg of histone H2B and compound. The mixture is incubated at 30°C for 5 min. The reaction is terminated by adding 1mL of ice-cold 20% trichloroacetic acid after adding 500 μg of bovine serum albumin as a carrier protein. The sample is centrifuged at 3000 r.p.m. for 15min, the pellet is resuspended in ice-cold 10% trichloro-acetic acid solution and the centrifugation-resuspension cycle is repeated three times. The final pellet is dissolved in 1 mL of 1 N NaOH and radioactivity is measured with a liquid scintillation counter.
Synonyms AT877, Fasudil hydrochloride, TWNT-4 cells, TIMP-1, AT-877, a-SMA, Protein kinase A, Ca channels, PKA, HIV, rat HSCs, HA 1077, Rho-associated protein kinase, human HSC, ERK1/2, Rho-kinase, inhibit, PKC, Ca2+ channels, HA1077, Rho-associated kinase, HA-1077 hydrochloride, Fasudil HCl, p38, lysophoaphatidic acid, Fasudil, Ca2+ channel antagonist, JNK, Calcium Channel, protein kinases, collagen, LPA, ROCK, Fasudil (HA-1077) HCl, Inhibitor, Protein kinase C, vasodilator, ROK, Human immunodeficiency virus, Autophagy, orally active, HA-1077, AT 877
Molecular Weight 327.83
Formula C14H18ClN3O2S
CAS No. 105628-07-7

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

H2O: 32.8 mg/mL (100 mM)

DMSO: 24.6 mg/mL (75 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Ono-Saito N, et al. Pharmacol Ther, 1999, 82(2-3), 123-131. 1. Sun L, Sun L, Li X, et al. A Novel Tigecycline Adjuvant ML-7 Reverses the Susceptibility of Tigecycline-Resistant Klebsiella pneumoniae. Frontiers in cellular and infection microbiology. 2022: 1341. 2. Asano T, et al. J Pharmacol Exp Ther, 1987, 241(3), 1033-1040. 3. Asano T, et al. Br J Pharmacol, 1989, 98(4), 1091-1100. 4. Negoro N, et al. Biochem Biophys Res Commun, 1999, 262(1), 211-215. 5. Fukushima M, et al. Liver Int, 2005, 25(4), 829-838.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Compound Library Autophagy Compound Library GPCR Compound Library Anti-Cardiovascular Disease Compound Library TGF-beta/Smad Compound Library Inhibitor Library Ion Channel Inhibitor Library DNA Damage & Repair Compound Library Neural Regeneration Compound Library Anti-Metabolism Disease Compound Library

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