Powder: -20°C for 3 years | In solvent: -80°C for 1 year
GSK923295 is a selective allosteric inhibitor of CENP-E kinesin motor ATPase(Ki=3.2 nM).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 45.00 | |
2 mg | In stock | $ 64.00 | |
5 mg | In stock | $ 98.00 | |
10 mg | In stock | $ 177.00 | |
25 mg | In stock | $ 349.00 | |
50 mg | In stock | $ 537.00 | |
100 mg | In stock | $ 775.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 138.00 |
Description | GSK923295 is a selective allosteric inhibitor of CENP-E kinesin motor ATPase(Ki=3.2 nM). |
Targets&IC50 | CENP-E:3.2 nM(Ki) |
Kinase Assay | Enzymology: Kinesin motor domains are expressed in Escherichia coli BL21(DE3) and purified. CENP-E proteins includes residues 2–340 with a carboxyl-terminal 6-his tag. All studies using MT are conducted in PEM25 buffer [25 mM PipesK+ (pH 6.8), 2 mM MgCl2, 1 mM EGTA] supplemented with 10 μM paclitaxel. The IC50 for steady-state inhibition is determined at 500 μM ATP, 5 μM MT, and 1 nM CENP-E in PEM25 buffer. |
Cell Research | Cell-growth inhibition assays are performed by MDS in 384-well plates, and DNA content of fixed cells stained with DAPI using an Incell 1000 is analyzed. DNA content is determined 24 h after seeding (T0) and after exposure to varying concentrations of drug for an additional 72 h (T72). All T72 measurements are normalized to T0. Curves are analyzed using the XLfit curve-fitting tool to determine the concentration of GSK923295 yielding 50% growth inhibition relative to T0 and Ymax values (GI50).(Only for Reference) |
Molecular Weight | 592.13 |
Formula | C32H38ClN5O4 |
CAS No. | 1088965-37-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 93 mg/mL (157.1 mM)
DMSO: 93 mg/mL (157.1 mM)
You can also refer to dose conversion for different animals. More
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GSK-923295 1088965-37-0 Apoptosis Cytoskeletal Signaling Kinesin GSK923295 inhibit Inhibitor GSK 923295 inhibitor