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Ginsenoside Rh3

Catalog No. TQ0199 CAS 105558-26-7

Ginsenoside Rh3 is a natural product extracted from Ginseng C. A. Mey.Ginsenoside Rh3 has antifungal and antioxidant activities and induces Nrf2 activation in human retinal cells.

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Ginsenoside Rh3, CAS 105558-26-7

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Description	Ginsenoside Rh3 is a natural product extracted from Ginseng C. A. Mey.Ginsenoside Rh3 has antifungal and antioxidant activities and induces Nrf2 activation in human retinal cells.
In vitro	Ginsenoside Rh3, at concentrations ranging from 0.01 to 10 μM, exhibits no cytotoxicity in tested models. In retinal pigment epithelium cells (RPEs), Ginsenoside Rh3 activates the Nrf2 pathway, as evidenced by dose-dependent increases in mRNA and protein levels of Nrf2-regulated genes, such as HO1, NQO1, and GCLC, following treatment. This enhancement in gene expression indicates a potentiation of cellular antioxidative mechanisms without altering Nrf2 mRNA levels, although Nrf2 protein levels significantly rise post-treatment with Ginsenoside Rh3 (3-10 μM). Additionally, the viability of SP 1-keratinocytes in response to Ginsenoside Rh3 has been evaluated using the EZ-Cytox assay, further delineating its safety and therapeutic potential.
In vivo	Ginsenoside Rh3 (5 mg/kg; intravitreal injection; 30 min pre-treatment) significantly attenuates light-induced decrease of both a- and b-wave amplitude. The electroretinography (ERG)'s a-wave decreases to 46.03±1.62% % of the control level after light exposure, which is back to 71.84±7.51% with Ginsenoside Rh3 administration. The b-wave is 40.19±3.34% of the control level by light exposure, and Rh3 intravitreal injection brings back to 80.01±2.37% of the control level.[1]
Cell Research	SP-1 keratinocytes are seeded in 96 well plates (2×10^4 cells/well). After 24 h, the media is replaced with media containing various concentrations of (A) SKRG, or (B) Ginsenoside Rh3 (0.01, 0.1, 1 and 10 μM). Control cells are treated with DMSO at a final concentration of 0.1%. After 24 h, the media containing the compounds or DMSO is replaced with media containing 10% EZ-Cytox. The cells are then incubated at 37°C for 1 h, and the absorbance is measured using a microplate reader at a wavelength of 450 nm. All assays are performed in triplicate [2].
Animal Research	The BALB/c mice (male, 5-6 week old, 17-18 g weight) are used. The pupillary dilation is performed before exposure to 5000 lx of white fluorescent light. Thirty min before light exposure, Ginsenoside Rh3 (at 5  mg/kg body weight) is injected intravitreally to the right eye. ERG recording after light exposure is also reported early. The b-wave amplitude is measured from the trough of the a-wave to the peak of the b-wave, and the amplitude of the a-wave is measured from the initial baseline [1].
Source	Natural Products > Araliaceae > Panax > Panax ginseng C. A. Mey.

Molecular Weight	604.86
Formula	C36H60O7
CAS No.	105558-26-7

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 25 mg/mL (41.33 mM)

DMSO: 3.02 mg/mL (5 mM), Sonification and heating are recommended.

References and Literature

1. Tang CZ, et al. Activation of Nrf2 by Ginsenoside Rh3 protects retinal pigment epithelium cells and retinal ganglion cells from UV. Free Radic Biol Med. 2018 Mar;117:238-246. 2. Chung I, et al. Inhibitory mechanism of Korean Red Ginseng on GM-CSF expression in UVB-irradiated keratinocytes. J Ginseng Res. 2015 Oct;39(4):322-30.

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Keywords

Ginsenoside Rh3 105558-26-7 Immunology/Inflammation Microbiology/Virology oxidation-reduction Antioxidant Antifungal Nrf2 Ginsenoside Rh-3 inhibitor inhibit

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