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Cell Cycle/Checkpoint Aurora Kinase Hesperadin

Hesperadin

Catalog No. T6532   CAS 422513-13-1

Hesperadin(IC50=250 nM) effectively inhibits Aurora B. It potently reduces the activity of AMPK, MAPKAP-K1, MKK1, Lck, CHK1 and PHK, but it could not inhibit MKK1 activity in vivo.

Hesperadin, CAS 422513-13-1
Pack Size Availability Price/USD Quantity
2 mg In stock 42.00
5 mg In stock 59.00
10 mg In stock 86.00
25 mg In stock 143.00
50 mg In stock 230.00
100 mg In stock 410.00
1 mL * 10 mM (in DMSO) In stock 66.00
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Biological Description
Chemical Properties
Storage & Solubility Information
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Description Hesperadin(IC50=250 nM) effectively inhibits Aurora B. It potently reduces the activity of AMPK, MAPKAP-K1, MKK1, Lck, CHK1 and PHK, but it could not inhibit MKK1 activity in vivo.
Targets&IC50 Aurora B (human) :ic50 250nM,   TbAUK1 :ic50 40nM,  
Kinase Assay For the Aurora B kinase assay, HeLa cells are lysed in a buffer containing 50 mM NaCl, then centrifuging at 13,000 rpm for 20 minutes at 4 °C. Discard supernatant, add 15 mL lysis buffer containing 250 mM NaCl in order to obtain active Aurora B kinase. Centrifuging at low-speed supernatant of the latter extract is used for immunoprecipitation. Monoclonal mouse anti–AIM-1, or mouse anti-HA, is coupled to GammaBind Plus Sepharose, and beads are rotated over-end in the extract for 90 minutes at 4 °C. Beads are washed, aliquoted, and washed in kinase buffer (20 mM Tris, pH 7.5, 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10 mM NaF). The kinase assay is performed with 10 μL beads in 20 μL kinase buffer containing 5 μg histone H3, 10 μM ATP, 2.5 μCi [γ-32P]ATP, and different concentrations of Hesperadin for 20 minutes at 37 °C.
Cell Research
HeLa cells and PtK1 cells are added Hesperadin 500 nM for 24 and 48 hours.
Cell lines: HeLa cells and PtK1 cells
Molecular Weight 516.65
Formula C29H32N4O3S
CAS No. 422513-13-1

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 55.3 mg/mL (100 mM)

Ethanol: 27.7 mg/mL (50 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

30% PEG400/0.5% Tween80/5% propylene glycol: 30 mg/mL

Citations

References and Literature
1. Hauf S, et al. J Cell Biol, 2003, 161(2), 281-294. 2. Jetton N, et al. Mol Microbiol, 2009, 72(2), 442-458. 3. Hu Y, et al. Chemical Genomics Approach Leads to the Identification of Hesperadin, an Aurora B Kinase Inhibitor, as a Broad-Spectrum Influenza Antiviral. Int J Mol Sci. 2017 Sep 8;18(9).

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Autophagy Compound Library Epigenetics Compound Library DNA Damage & Repair Compound Library Kinase Inhibitor Library Cell cycle related Compound Library

Related Products

Related compounds with same targets
SP-146 Aurora Kinase Inhibitor III SNS-314 Mesylate Aurora inhibitor 1 SCH-1473759 AZD1152 MBM-17S MBM-17

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