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Hydroxyfasudil Hydrochloride

Catalog No. T4276 CAS 155558-32-0

Synonyms: Hydroxyfasudil (HA-1100) HCl, HA 1100 hydrochloride

Hydroxyfasudil Hydrochloride (HA 1100 hydrochloride) is a ROCK1/2 inhibitor (IC50s: 0.73/0.72 μM).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Hydroxyfasudil Hydrochloride Chemical Structure

Hydroxyfasudil Hydrochloride, CAS 155558-32-0

Pack Size	Availability	Price/USD
2 mg	In stock	$ 34.00
5 mg	In stock	$ 55.00
10 mg	In stock	$ 84.00
25 mg	In stock	$ 127.00
50 mg	In stock	$ 155.00
100 mg	In stock	$ 288.00
1 mL * 10 mM (in DMSO)	In stock	$ 61.00

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Purity: 99.82%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Hydroxyfasudil Hydrochloride (HA 1100 hydrochloride) is a ROCK1/2 inhibitor (IC50s: 0.73/0.72 μM).
Targets&IC50	ROCK1:0.73 μM, ROCK2:0.72 μM
In vitro	Hydroxyfasudil has a weak inhibition of PKA (IC50: 37 μM), 50-fold higher than those of the ROCKs. Hydroxyfasudil increases eNOS mRNA levels (EC50: 0.8 ± 0.3 μM). Hydroxyfasudil (0-100 μM) concentration-dependently increases eNOS activity and stimulates NO production in HAEC. Hydroxyfasudil (10 μM) increases the half-life of eNOS mRNA from 13 to 16 hours but does not affect eNOS promoter activity (0.1-100 μM).
In vivo	Hydroxyfasudil (10 mg/kg, i.p.) markedly increases both the maximal and average voided volumes in SD rats. Hydroxyfasudil also markedly reduces the maximal detrusor pressure. In spontaneously hypertensive rats (SHRs), Hydroxyfasudil (3 mg/kg, i.p.) inhibits hypercontractility induced by norepinephrine. Additionally, Hydroxyfasudil (3/10 mg/kg, i.p.) significantly ameliorates decreased penile cGMP contents in rats.
Animal Research	Hydroxyfasudil is formulated in saline.Micturition behavior is studied after administration of either Hydroxyfasudil (10 mg/kg, i.p.) or a corresponding volume of saline. Each rat is placed in a metabolic cage containing a urine collection funnel that is placed over an electronic balance. The balance is connected to a personal computer via a multiport controller and used to measure the cumulative weight of the collected urine. Every 150 s during a continuous 24-h period, the computer samples and records the data for the micturition frequency and volumes. The micturition reflex parameters that are collected include: urine volume per micturition, maximal micturition volume, micturition frequency, and total urine output in the Hydroxyfasudil- or vehicle-treated animals. Each monitoring session started at 18.00 hours. Prior to being placed in the metabolic cage at the start of each experimental period, the animals received either a single injection of Hydroxyfasudil (10 mg/kg) dissolved in saline or an injection of saline without the inhibitor.

Synonyms	Hydroxyfasudil (HA-1100) HCl, HA 1100 hydrochloride
Molecular Weight	343.83
Formula	C14H18ClN3O3S
CAS No.	155558-32-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 62 mg/mL(180.3 mM)

References and Literature

1. Rikitake Y, et al. Inhibition of Rho kinase (ROCK) leads to increased cerebral blood flow and stroke protection. Stroke. 2005 Oct;36(10):2251-7. Epub 2005 Sep 1. 2. Masago T, et al. Effect of the rho-kinase inhibitor hydroxyfasudil on bladder overactivity: an experimental rat model. Int J Urol. 2009 Oct;16(10):842-7. 3. Saito M, et al. Hydroxyfasudil ameliorates penile dysfunction in the male spontaneously hypertensive rat. Pharmacol Res. 2012 Oct;66(4):325-31.

Citations

1. Sun L, Sun L, Li X, et al. A Novel Tigecycline Adjuvant ML-7 Reverses the Susceptibility of Tigecycline-Resistant Klebsiella pneumoniae. Frontiers in cellular and infection microbiology. 2022: 1341.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library TGF-beta/Smad Compound Library Cytoskeletal Signaling Pathway Compound Library Stem Cell Differentiation Compound Library Kinase Inhibitor Library Reprogramming Compound Library Anti-Aging Compound Library Bioactive Compound Library NO PAINS Compound Library Immunology/Inflammation Compound Library

Related Products

Related compounds with same targets

Y-9738 CRT0066854 hydrochloride SR-3677 ROCK-IN-7 ROCK-IN-6 Rho-Kinase-IN-1 ZINC00881524 CCG-232964

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Hydroxyfasudil Hydrochloride 155558-32-0 Cell Cycle/Checkpoint Cytoskeletal Signaling Stem Cells ROCK Hydroxyfasudil (HA-1100) HCl HA-1100 Hydroxyfasudil Rho-associated kinase HA 1100 inhibit HA1100 HA1100 Hydrochloride Rho-kinase Rho-associated protein kinase HA-1100 Hydrochloride ROK HA 1100 hydrochloride Inhibitor HA 1100 Hydrochloride inhibitor

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