Powder: -20°C for 3 years | In solvent: -80°C for 1 year
INH1 (IBT13131) is a cell-permeable Hec1 inhibitor that specifically disrupts the Hec1/Nek2 interaction.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
10 mg | In stock | $ 39.00 | |
25 mg | In stock | $ 81.00 | |
50 mg | In stock | $ 147.00 | |
100 mg | In stock | $ 239.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 29.00 |
Description | INH1 (IBT13131) is a cell-permeable Hec1 inhibitor that specifically disrupts the Hec1/Nek2 interaction. |
Targets&IC50 | Hec1:10-21 μM |
In vitro | Intraperitoneal injection of 100 mg/kg INH1 inhibits the growth of mammary tumors in mice with MDA-MB-468 human breast cancer xenografts. |
In vivo | INH1 effectively inhibits the proliferation of human breast cancer cells with a GI50 of 10-21 μM. Additionally, INH1 induces cell-killing activity by disrupting the spindle checkpoint-regulated Hec1/Nek2 pathway. |
Kinase Assay | Binding assays: Surface plasma resonance (SPR) assays are performed at 22.5°C in HBSD buffer [10 mmol/L HEPES, 150 mmol/L NaCl, 0.1% DMSO (pH 7.5)] on Biacore 3000. 6×His-Hec1 and GST-Nek2 are purified. NTA sensor chip or glutathione-modified CM5 chip are used to capture His-Hec1 and GST-Nek2, respectively. The capture level is about 140 to 180 resonance units (RU) at the flow rate of 5 μL/min. For the binding assay, chips are sequentially treated with compounds (1 or 20 μmol/L) and then proteins (50 μg/mL). Retained RUs are recorded and processed (triplicate experiments). |
Cell Research | Standard 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays with a 3-d drug treatment procedure are performed to measure the dose-dependent cytotoxicity of INH1 in cultured cells. Triplicate sets are measured and compiled for final data presentation.(Only for Reference) |
Synonyms | IBT13131 |
Molecular Weight | 308.4 |
Formula | C18H16N2OS |
CAS No. | 313553-47-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30.8 mg/mL (100 mM)
Ethanol: 3.1 mg/mL (10 mM)
You can also refer to dose conversion for different animals. More
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INH1 313553-47-8 Apoptosis Cytoskeletal Signaling Microtubule Associated inhibit INH 1 Inhibitor IBT-13131 INH-1 IBT13131 IBT 13131 inhibitor