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Inauhzin

Catalog No. T1887 CAS 309271-94-1

Synonyms: INZ

Inauhzin (INZ)(INZ) is a novel small molecule that effectively reactivates p53 by inhibiting SIRT1 activity, promotes p53-dependent apoptosis of human Y cells without causing apparently genotoxic stress(IC50=3 uM, in A549 cell).

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Inauhzin, CAS 309271-94-1

Pack Size	Availability	Price/USD
1 mg	In stock	$ 37.00
2 mg	In stock	$ 51.00
5 mg	In stock	$ 91.00
10 mg	In stock	$ 112.00
25 mg	In stock	$ 238.00
50 mg	In stock	$ 408.00
100 mg	In stock	$ 585.00
500 mg	In stock	$ 1,270.00
1 mL * 10 mM (in DMSO)	In stock	$ 98.00

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Purity: 99.36%

Purity: 98%

Purity: 97.69%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Inauhzin (INZ)(INZ) is a novel small molecule that effectively reactivates p53 by inhibiting SIRT1 activity, promotes p53-dependent apoptosis of human Y cells without causing apparently genotoxic stress(IC50=3 uM, in A549 cell).
Targets&IC50	SIRT1:3 μM
Kinase Assay	Kinase Profile Assay: Assays for serine/threonine kinases using radio labeled [γ-33P] ATP are performed in 96 well plates. BRAF and c-RAF are expressed as N-terminal FLAG-tagged protein using a baculovirus expression system. The reaction conditions are optimized for each kinase: BRAF (25 ng/well of enzyme, 1 μg/well of GST-MEK1(K96R), 0.1 μCi/well of [γ-32P] ATP, room temperature, 20 min reaction); c-RAF (25 ng/well of enzyme, 1 μg/well of GST-MEK1 (K96R), 0.1 μCi/well of [γ-32P] ATP, room temperature, 20 min reaction). Enzyme reactions are performed in 25 mM HEPES, pH 7.5, 10 mM magnesium acetate, 1 mM dithiothreitol and 0.5 μM ATP containing optimized concentration of enzyme, substrate and radiolabeled ATP as described above in a total volume of 50 μL. Prior to the kinase reaction, compound and enzyme are incubated for 5 min at reaction temperature as described above. The kinase reactions are initiated by adding ATP. After the reaction period as described above, the reactions are terminated by the addition of 10% (final concentration) trichloroacetic acid. The [γ-33P] or [γ-32P]-phosphorylated proteins are filtered in GFC filter plates with a Cell Harvester and then the plates are washed out with 3% phosphoric acid. The plates are dried, followed by the addition of 40 μL of MicroScint0. The radioactivity is counted by a TopCount scintillation counter.

Synonyms	INZ
Molecular Weight	469.58
Formula	C25H19N5OS2
CAS No.	309271-94-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 21 mg/mL

References and Literature

1. Zhang Q, et al. EMBO Mol Med. 2012 Apr; 4(4): 298-31

Citations

1. Sun Y, Yang Y M, Hu Y Y, et al. Inhibition of nuclear deacetylase Sirtuin-1 induces mitochondrial acetylation and calcium overload leading to cell death. Redox Biology. 2022: 102334 2. Sun Y, Yang Y M, Hu Y Y, et al. Inhibition of nuclear deacetylase Sirtuin-1 induces mitochondrial acetylation and calcium overload leading to cell death. Redox Biology. 2022: 102334

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Apoptosis Compound Library Reprogramming Compound Library Anti-Colorectal Cancer Compound Library Epigenetics Compound Library NO PAINS Compound Library Ferroptosis Compound Library Transcription Factor-Targeted Compound Library Target-Focused Phenotypic Screening Library Stem Cell Differentiation Compound Library

Related Products

Related compounds with same targets

SIRT1 activator 1 YM-08 N-Caffeoyltryptophan YK-3-237 SIRT5 inhibitor 9 Thiomyristoyl Sirtuin modulator 1 Suramin Sodium Salt

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Keywords

Inauhzin 309271-94-1 Chromatin/Epigenetic DNA Damage/DNA Repair Sirtuin Inhibitor MDM-2/p53 inhibit INZ inhibitor

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