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Metabolism Lipase JZL184

JZL184

Catalog No. T6554   CAS 1101854-58-3
Purity 97.89% Datasheet MSDS

JZL 184 is a potent and selective inhibitor of MAGL with IC50 of 8 nM and 4 μM for inhibition of MAGL and FAAH in mouse brain membranes respectively.

JZL184, CAS 1101854-58-3
Pack Size Availability Price/USD Quantity
10 mg In stock 53.00
25 mg In stock 106.00
100 mg In stock 404.00
200 mg In stock 727.00
1 mL * 10 mM (in DMSO) In stock 55.00
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Biological Description
Chemical Properties
Storage & Solubility Information
Preparing Solutions
Description JZL 184 is a potent and selective inhibitor of MAGL with IC50 of 8 nM and 4 μM for inhibition of MAGL and FAAH in mouse brain membranes respectively.
Targets&IC50 MAGL :ic50 8nM,  
Kinase Assay activity-based protein profiling (ABPP): Mouse brains are Dounce-homogenized in PBS, pH7.5, followed by a low-speed spin (1,400×, 5 min) to remove debris. The supernatant is then subjected to centrifugation (64,000×, 45 min) to provide the cytosolic fraction in the supernatant and the membrane fraction as a pellet. The pellet is washed and resuspended in PBS buffer by sonication. Total protein concentration in each fraction is determined using a protein assay kit. Samples are stored at -80 °C until use. Mouse brain membrane proteomes, are diluted to 1 mg/mL in PBS and pre-incubated with varying concentrations of inhibitors (1 nM to 10 mM) for 30 min at 37 °C before the addition of FP-rhodamine at a final concentration of 2 mM in a 50 mL total reaction volume. After 30 min at 25 °C, the reactions are quenched with 4×SDS-PAGE loading buffer, boiled for 5 min at 90 °C, subjected to SDS-PAGE and visualized in-gel using a flatbed fluorescence s
Cell Research
1 × 105 cells are split into four-well chamber slides and incubated with culture medium containing BrdU for 4 h. BrdU staining is performed following the manufacturer’s instructions.(Only for Reference)
Cell lines: LOVO, Caco-2, SW480
Animal Research
Animal Model: C57Bl/6 mice
Appearance solid
Molecular Weight 520.49
Formula C27H24N2O9
CAS No. 1101854-58-3

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 93 mg/mL (178.7 mM)

Ethanol: <1 mg/mL

Water: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Solution 1

30% PEG400/0.5% Tween80/5% propylene glycol: 30 mg/mL

Citations

References and Literature
1. Long JZ, et al. Nat Chem Biol, 2009, 5(1), 37-44. 2. Ye L, et al. Cancer Lett, 2011, 307(1), 6-17.

Related Compound Libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Bioactive Lipid Compound Library Anti-Metabolism Disease Compound Library Anti-cancer Metabolism Compound Library

Related Products

Related compounds with same targets
Endothelial lipase inhibitor-1 ABX-1431 JZL195 JJKK 048 Beta-Sitosterol KML29 Atglistatin XEN-445

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