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Ketorolac tromethamine salt

Catalog No. T1212   CAS 74103-07-4
Synonyms: Acular LS, Toradol, Ketorolac tris salt, Acular

Ketorolac tromethamine salt (Ketorolac tris salt) non-selective inhibits the enzymes cyclooxygenase 1 (COX-1) and COX-2with anti-inflammatory, analgesic, and antipyretic activities.

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Ketorolac tromethamine salt Chemical Structure
Ketorolac tromethamine salt, CAS 74103-07-4
Pack Size Availability Price/USD Quantity
500 mg In stock $ 46.00
1 g In stock $ 79.00
1 mL * 10 mM (in DMSO) In stock $ 51.00
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Purity: 99.85%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Ketorolac tromethamine salt (Ketorolac tris salt) non-selective inhibits the enzymes cyclooxygenase 1 (COX-1) and COX-2with anti-inflammatory, analgesic, and antipyretic activities.
Targets&IC50 COX1:20 nM, COX2:20 nM
Kinase Assay Inhibition of Prostaglandin Formation: Recombinant COX-1 and COX-2 from rat (rCOX) and human (hCOX) expressed in a baculovirus system are purified and reconstituted with 2 mM phenol and 1 μM hematin. Then the cyclooxygenase activity is measured using a radiometric assay, and the specific activity of the final enzyme preparations used is between 20,000 and 35,000 units. Ketorolac (2 -15 μL) are diluted in DMSO and preincubated with the appropriate recombinant COX (3 -15 ng) at a final concentration of 0.01 to 1000 μM in a reaction mixture (150 μL) containing 50 mM Tris-HCl buffer (pH 7.9), 2 mM EDTA, 10% glycerol, 2 mM phenol, and 1 μM hematin for 10 minutes. The reaction is initiated by addition of [14C]arachidonic acid (50–60 mCi/mmol in a final concentration of 20 μM) and is terminated 45 seconds later by the addition of 100 μL of 0.2 N HCl and 750 μL of distilled water. The total reaction volume is then applied to a 1 mL C18 Sep-pak column that has previously been washed with 2 mL of methanol followed by 5 mL of deionized water. Oxygenated products are eluted with 3 mL of a mixture of acetonitrile/water/acetic acid (50:50:0.1, v/v/v) and quantified by liquid scintillation spectroscopy.
Cell Research Human osteoblasts cells are exposed to Ketorolac for 24 hours. Thymidine incorporation is assessed by the TopCount Microplate Scintillation and Luminescence Counters through adding [3H]-thymidine to cultures 4 hours prior to harvesting. Cell cycle distribution is determined by using propidium iodide in flow cytometer, and cell apoptosis or necrosis is detected using the Annexin V-FITC Apoptosis Detection Kit. (Only for Reference)
Synonyms Acular LS, Toradol, Ketorolac tris salt, Acular
Molecular Weight 376.4
Formula C19H24N2O6
CAS No. 74103-07-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 70 mg/mL (186 mM)

H2O: 14 mg/mL(37.2 mM)

Ethanol: 70 mg/mL (186 mM)

TargetMolReferences and Literature

1. Jett MF, et al. J Pharmacol Exp Ther, 1999, 288(3), 1288-1297. 2. Berg J, et al. Inflamm Res, 1999, 48(7), 369-379. 3. Chang JK, et al. Toxicology, 2009, 258(2-3), 148-156. 4. Wallace JL, et al. Gastroenterology, 2000, 119(3), 706-714.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Clinical Compound Library Anti-Neurodegenerative Disease Compound Library Anti-Cancer Drug Library Anti-Cancer Approved Drug Library Angiogenesis related Compound Library Pyroptosis Compound Library Bioactive Compounds Library Max Clinical Compound Library Drug-Fragment Library Orally Active Compound Library

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Keywords

Ketorolac tromethamine salt 74103-07-4 Immunology/Inflammation Neuroscience COX Cyclooxygenase RS37619 RS-37619 Ketorolac tromethamine Acular LS Inhibitor inhibit Ketorolac tris Toradol RS 37619 Ketorolac tris salt Acular Ketorolac inhibitor

 

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