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L-NAME hydrochloride

Catalog No. T6570 CAS 51298-62-5

Synonyms: L-NAME HCl, N(G)-Nitro-L-arginine methyl ester, NG-Nitroarginine methyl ester hydrochloride

L-NAME hydrochloride (L-NAME HCl), a cell-permeable NO synthase inhibitor, displays Ki values of 4.4 μM, 15 nM, and 39 nM, for iNOS (mouse) , nNOS (bovine) , and eNOS (human), respectively.

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L-NAME hydrochloride, CAS 51298-62-5

Pack Size	Availability	Price/USD
100 mg	In stock	$ 46.00
200 mg	In stock	$ 54.00
500 mg	In stock	$ 76.00
1 mL * 10 mM (in DMSO)	In stock	$ 30.00

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Purity: 100%

Purity: 99.7%

Purity: 99.08%

Purity: 98.71%

Purity: 98.05%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	L-NAME hydrochloride (L-NAME HCl), a cell-permeable NO synthase inhibitor, displays Ki values of 4.4 μM, 15 nM, and 39 nM, for iNOS (mouse) , nNOS (bovine) , and eNOS (human), respectively.
Targets&IC50	nNOS:15 nM(Ki), eNOS:39 nM(Ki)
In vivo	L-NAME (0.03-300 mg kg-1, i.v.) induces a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. L-NAME (100 mg kg-1, i.v.) inhibits significantly the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by L-NAME is reversed by L-arginine (30-100 mg kg-1, i.v.) in a dose-dependent manner. [2]
Kinase Assay	Enzyme Assay: The oxidation of L-arginine is monitored by the conversion of [3H]- or [14C]-arginine to L-citrulline which separates L-citrulline from L-arginine by Dowex 50x8-200 (Na) chromatography. Typical reaction mixtures (100 pL) contains 50 mM HEPES, pH 7.0, 8 pM tetrahydrobiopterin, 1 mM CaC12, 0.01 mg/mL calmodulin, 0.5 mM EDTA, 0.450 pM [14C]-arginine (30000 cpm), and 100-200 pM NADPH. The cNOS-catalyzed oxidation of NADPH to NADP+ is monitored by the reduction of absorbance at 340 nm with a Kontron 860 spectrophotometer in a volume of 300 pL. All reactions are at 30 ℃ unless otherwise indicated.
Cell Research	rMC-1 cells are incubated in 5 or 25 mM glucose, with or without l-NAME (1 mM). Media is changed every other day for up to 5 days. BREC cells are incubated in 5 or 25 mM glucose as well as inhibitor as described above for 5 days. Cell death is determined by light microscopy using a hemocytometer and a 0.4% trypan blue dye exclusion method. The number of cells that do not exclude the dye is expressed per 1,000 total cells. A minimum of 800 cells is counted per assay (8 dishes, >100 cells counted per dish), and the assay is replicated three times on different days. (Only for Reference)

Synonyms	L-NAME HCl, N(G)-Nitro-L-arginine methyl ester, NG-Nitroarginine methyl ester hydrochloride
Molecular Weight	269.69
Formula	C7H15N5O4·HCl
CAS No.	51298-62-5

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 27 mg/mL (100 mM)

H2O: 27 mg/mL (100 mM)

References and Literature

1. Furfine ES, et al. Biochemistry, 1993, 32(33), 8512-8517. 2. Rees DD, et al. Br J Pharmacol, 1990, 101(3), 746-752. 3. Du Y, et al. J Physiol Regul Integr Comp Physiol, 2004, 287(4), R735-R741. 4. Heggers JP, et al. J Altern Complement Med, 1997, 3(2), 149-153.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Drug Repurposing Compound Library NO PAINS Compound Library Bioactive Compound Library Anti-Aging Compound Library Nonsteroidal Anti-Inflammatory Compound Library Clinical Compound Library Bioactive Compounds Library Max Immunology/Inflammation Compound Library

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Keywords

L-NAME hydrochloride 51298-62-5 Immunology/Inflammation NO Synthase NOS L-NAME HCl inhibit NG-Nitroarginine methyl ester Hydrochloride Inhibitor L-NAME Nitric oxide synthases L-NAME Hydrochloride LNAME hydrochloride L NAME hydrochloride NG-Nitroarginine methyl ester N(G)-Nitro-L-arginine methyl ester NG-Nitroarginine methyl ester hydrochloride inhibitor

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