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ML335

Catalog No. T4224 CAS 825658-06-8

ML335 is a selective activator of TREK-1 and TREK-2.

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ML335, CAS 825658-06-8

Pack Size	Availability	Price/USD
5 mg	In stock	$ 57.00
10 mg	In stock	$ 96.00
25 mg	In stock	$ 210.00
50 mg	In stock	$ 347.00
100 mg	In stock	$ 513.00
500 mg	In stock	$ 1,080.00
1 mL * 10 mM (in DMSO)	In stock	$ 57.00

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Purity: 99.73%

Purity: 99.42%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	ML335 is a selective activator of TREK-1 and TREK-2.
In vitro	Xenopus oocyte two-electrode voltage-clamp measurements show that ML335 and ML402 activate K2P2.1 and K2P10.1 but not K2P4.1 (14.3±2.7?μM, K2P2.1-ML335; 13.7±7.0?μM, K2P2.1-ML402; 5.2±0.5?μM, K2P10.1-ML335; and 5.9±1.6?μM, K2P10.1-ML402). Swapping the Lys271 equivalent between K2P2.1 and K2P4.1 results in a clear phenotype reversal for ML335 and M402 activation. ML335 and ML402 activate K2P2.1 in HEK293 cells similar to their effects in Xenopus oocytes (5.2±0.8?μM and 5.9±1.6?μM for ML335 and ML402, respectively (n≥3))
Cell Research	Mouse K2P2.1, human K2P4.1, and mutants are expressed from a previously described pIRES2-EGFP vector in HEK293T cells (ATTC). 70% confluent cells are transfected (in 35-mm diameter wells) with LipofectAMINE 2000 for 6?h, and plated onto coverslips coated with Matrigel. Effects of ML335, ML402 and arachidonic acid on K2P2.1 current at 0?mV are measured by whole-cell patch-clamp experiments 24?h after transfection. Acquisition and analysis are performed using pCLAMP9 and an Axopatch 200B amplifier. Pipette resistance ranges from 1 to 1.5?MΩ. Pipette solution contains the following: 145?mM KCl, 3?mM MgCl2, 5?mM EGTA and 20?mM HEPES (pH 7.2 with KOH). Bath solution contains the following: 145?mM NaCl, 5?mM KCl, 1?mM CaCl2, 3?mM MgCl2 and 20?mM HEPES (pH 7.4 with NaOH). K2P2.1 currents are elicited by a 1?s ramp from -100 to +50?mV from a -80?mV holding potential. After stabilization of the basal current, ML335 and ML402 are perfused at 200?mL per hour until potentiation is stably reached[1].

Molecular Weight	373.25
Formula	C15H14Cl2N2O3S
CAS No.	825658-06-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 155 mg/mL

References and Literature

1. Lolicato M, et al. K2P2.1 (TREK-1)-activator complexes reveal a cryptic selectivity filter binding site. Nature. 2017 Jul 20;547(7663):364-368.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Compound Library Ion Channel Inhibitor Library Bioactive Compound Library NO PAINS Compound Library Potassium Channel Blocker Library Bioactive Compounds Library Max

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Keywords

ML335 825658-06-8 Membrane transporter/Ion channel Potassium Channel ML-335 ML 335 KcsA Inhibitor inhibit inhibitor

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