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MMAF

Catalog No. T3256 CAS 745017-94-1

Synonyms: MonoMethyl auristatin F

MMAF (MonoMethyl auristatin F), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

MMAF, CAS 745017-94-1

Pack Size	Availability	Price/USD
2 mg	In stock	$ 39.00
5 mg	In stock	$ 64.00
10 mg	In stock	$ 97.00
25 mg	In stock	$ 147.00
50 mg	In stock	$ 198.00
100 mg	In stock	$ 278.00
200 mg	In stock	$ 418.00
1 mL * 10 mM (in DMSO)	In stock	$ 98.00

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Purity: 98.48%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	MMAF (MonoMethyl auristatin F), an antimitotic agent, inhibits cell division by blocking the polymerization of tubulin.
Targets&IC50	H3397:105 nM.
In vitro	MMAF shows in vitro cytotoxicity against a panel of cell lines. The IC50 values for Karpas 299, H3396, 786-O and Caki-1 are 119, 105, 257, and 200 nM, respectively. Targeted MMAF is much more potent than the free drug, and that cAC10 conjugates of MMAF display pronounced activities. On a molar basis, the cAC10-L1-MMAF4 is an average of over 2200-fold more potent than free MMAF and is active on all the CD30-positive cell lines tested[1].
In vivo	The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF4 has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF4 ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively[1].
Cell Research	Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay[2].

Synonyms	MonoMethyl auristatin F
Molecular Weight	731.96
Formula	C39H65N5O8
CAS No.	745017-94-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 20 mg/mL (27.32 mM)

References and Literature

1. Doronina SO, et al. Enhanced activity of monomethylauristatin F through monoclonal antibody delivery: effects of linker technology on efficacy and toxicity. Bioconjug Chem. 2006 Jan-Feb;17(1):114-24.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Microtubule-Targeted Compound Library Drug-induced Liver Injury (DILI) Compound Library Anti-Cancer Compound Library NO PAINS Compound Library Cytoskeletal Signaling Pathway Compound Library Anti-COVID-19 Compound Library Bioactive Compounds Library Max Human Metabolite Library Bioactive Compound Library

Related Products

Related compounds with same targets

Tubulysin A MAP4343 Plinabulin Myoseverin Lexibulin Taccalonolide A Tubulin inhibitor 35 Cabazitaxel

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

MMAF 745017-94-1 Cytoskeletal Signaling Others Microtubule Associated Monomethylauristatin F inhibit Microtubule/Tubulin ADC Payload ADC Cytotoxin Inhibitor MonoMethyl auristatin F inhibitor

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