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NSC-87877 disodium

Catalog No. T6911 CAS 56932-43-5

Synonyms: NSC87877

NSC-87877 disodium (NSC87877) is a cell-permeable inhibitor of both SHP-1 and SHP-2 with IC50 values of 355 and 318 nM respectively. It also inhibits EGF-induced Erk1/2 activation in HEK293 cells and significantly reduces MDA-MB-468 cell viability/proliferation.

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NSC-87877 disodium, CAS 56932-43-5

Pack Size	Availability	Price/USD
5 mg	In stock	$ 65.00
10 mg	In stock	$ 94.00
25 mg	In stock	$ 195.00
50 mg	In stock	$ 326.00
100 mg	In stock	$ 486.00
200 mg	In stock	$ 695.00
1 mL * 10 mM (in DMSO)	In stock	$ 83.00

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Purity: 98%

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Description	NSC-87877 disodium (NSC87877) is a cell-permeable inhibitor of both SHP-1 and SHP-2 with IC50 values of 355 and 318 nM respectively. It also inhibits EGF-induced Erk1/2 activation in HEK293 cells and significantly reduces MDA-MB-468 cell viability/proliferation.
Targets&IC50	SHP2:318 nM, SHP1:355 nM
In vitro	NSC-87877 seems to have no selectivity between human Shp2 and Shp1 in vitro. NSC-87877 inhibits EGF-stimulated Shp2 activation and EGF-induced Erk1/2 activation. NSC-87877 suppresses Erk1/2 activation by a Gab1-Shp2 chimera but does not affect PMA(phorbol 12-myristate 13-acetate)-induced Erk1/2 Activation. NSC-87877 does not inhibit EGF-induced Gab1 tyrosine phosphorylation and Gab1-Shp2 association. NSC-87877 can significantly reduce MDA-MB-468 cell viability/proliferation, especially in combination with a phosphoinositide 3-kinase inhibitor[1].
In vivo	NSC-87877 almost completely prevented EAE(experimental autoimmune encephalomyelitis) development by blocking the accumulation of inflammatory cells in the CNS(central nervous system)[2]. In an intrarenal mouse model of neuroblastoma, NSC-87877 treatment results in decreased tumor growth and increased p53 and p38 activity[3]. Intrathecal administration of NSC-87877 (an SHP2 antagonist; 1, 10, or 100 μM/rat) into the allodynic rats suppresses spinal nerve ligation-induced allodynia, spinal SHP2 and NR2B phosphorylation, and SHP2/phosphorylated SHP2-PSD-95 and PSD-95-NR2B/phosphorylated NR2B coprecipitation[4].
Cell Research	Serum-starved cells are pretreated with NSC-87877 (50 μM,3 h) or dimethyl sulfoxide (solvent) and then stimulated with EGF (100 ng/ml, 5 min) or left untreated. Cells are lysed in ice-cold PTP(protein tyrosine phosphatase) lysis buffer. Shp2 or Shp1 in cell lysate supernatants (0.5 mg/each) is immunoprecipitated with an antibody to Shp2 or an antibody to Shp1 plus protein A-Sepharose for 2 h at 4°C. (Only for Reference)

Synonyms	NSC87877
Molecular Weight	503.42
Formula	C19H13N3O7S2·2Na
CAS No.	56932-43-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 93 mg/mL (184.7 mM)

H2O: 92 mg/mL (182.7 mM)

References and Literature

1. Chen L, et al. Mol Pharmacol. 2006, 70(2):562-70. 2. Luo Q, et al. Br J Pharmacol. 2014, 171(7):1706-21. 3. Shi Y, et al. Cell Death Dis. 2015, 6:e1841. 4. Peng HY, et al. Pain. 2012, 153(5):1042-53.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Phosphatase Inhibitor Library Lipid Metabolism Compound Library Apoptosis Compound Library Metabolism Compound Library Bioactive Compounds Library Max Bioactive Compound Library Immunology/Inflammation Compound Library Glycometabolism Compound Library

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Keywords

NSC-87877 disodium 56932-43-5 Apoptosis Metabolism Phosphatase Neuroblastoma inhibit NSC 87877 Inhibitor NSC 87877 disodium PPAR p53 NSC-87877 HSP27 NSC87877 disodium NSC87877 p38 inhibitor

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