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Nutlin-3a

Catalog No. T6023 CAS 675576-98-4

Synonyms: (-)-Nutlin-3, Nutlin-3a chiral, (−)-Nutlin-3

Nutlin-3a ((−)-Nutlin-3), the active enantiomer of Nutlin-3, inhibits MDM2-p53 interactions and stabilizes the p53 protein.

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Nutlin-3a, CAS 675576-98-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 45.00
2 mg	In stock	$ 64.00
5 mg	In stock	$ 97.00
10 mg	In stock	$ 167.00
25 mg	In stock	$ 282.00
50 mg	In stock	$ 418.00
100 mg	In stock	$ 618.00
1 mL * 10 mM (in DMSO)	In stock	$ 137.00

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Purity: 98.73%

Purity: 95.62%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Nutlin-3a ((−)-Nutlin-3), the active enantiomer of Nutlin-3, inhibits MDM2-p53 interactions and stabilizes the p53 protein.
Targets&IC50	MDM2-p53 interaction:90 nM
Kinase Assay	Biacore studies: Competition assays are performed on a Biacore S51. A Series S Sensor chip CM5 is derivatized for immobilization of a PentaHis antibody for capture of the His-tagged p53. The level of capture is ~ 200 response units (1 response unit corresponds to 1 pg of protein per mm 2). The concentration of MDM2 protein is kept constant at 300 nM. Test compounds are dissolved in DMSO at 10 mM and further diluted to make a concentration series of inhibitor in each MDM2 test sample. The assays are run at 25 °C in running buffer (10 mM Hepes, 0.15 M NaCl, 2% DMSO). MDM2-p53 binding in the presence of inhibitor is calculated as a percentage of binding in the absence of inhibitor and IC50 is calculated using Microsoft Excel
Cell Research	SRB(Only for Reference)

Synonyms	(-)-Nutlin-3, Nutlin-3a chiral, (−)-Nutlin-3
Molecular Weight	581.49
Formula	C30H30Cl2N4O4
CAS No.	675576-98-4

Storage

store at low temperature,keep away from direct sunlight

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 58.2 mg/mL (100 mM)

DMSO: 58.2 mg/mL (100 mM)

References and Literature

1. Tovar C, et al. Proc Natl Acad Sci U S A, 2006, 103(6), 1888-1893. 2. Ohnstad HO, et al. BMC Cancer, 2011, 11(211), 1-11. 3. Vassilev LT, et al. Science, 2004, 303(5659), 844-848.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Anti-Cancer Active Compound Library Target-Focused Phenotypic Screening Library Anti-Ovarian Cancer Compound Library Bioactive Compounds Library Max Anti-Pancreatic Cancer Compound Library Ubiquitination Compound Library Glycometabolism Compound Library PPI Inhibitor Library Glutamine Metabolism Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Nutlin-3a 675576-98-4 Apoptosis Autophagy Ubiquitination E1/E2/E3 Enzyme E1 activating enzyme inhibit E3 ligating enzyme p53 carcinomas ovarian (-)-Nutlin-3 Nutlin-3 Ubiquitin ligase Ubiquitin conjugating enzyme Ubiquitin activating enzyme Nutlin 3a Inhibitor Nutlin3a Nutlin-3a chiral MDM-2/p53 (−)-Nutlin-3 Rebemadlin E2 conjugating enzyme inhibitor

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