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Apoptosis PD-1/PD-L1 PD1-PDL1 inhibitor 2

PD1-PDL1 inhibitor 2

Catalog No. T3146   CAS 1675203-84-5
Synonyms: BMS-202, PD-1/PD-L1 inhibitor 2
Purity 98.54% Datasheet MSDS

PD1-PDL1 inhibitor 2 is an inhibitor of the PD-1 (Programmed death- 1) /PD-Ll (Programmed death-ligand 1) protein/protein interaction, extracted from patent WO/2015034820 A1.

PD1-PDL1 inhibitor 2, CAS 1675203-84-5
Pack Size Availability Price/USD Quantity
2 mg In stock 64.00
5 mg In stock 114.00
10 mg In stock 182.00
25 mg In stock 359.00
50 mg In stock 642.00
100 mg In stock 1100.00
1 mL * 10 mM (in DMSO) In stock 121.00
Bulk Size: Please Quote for Discount
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Purity 97.92%
HNMR HPLC COA Datasheet MSDS
Purity 98.54%
HNMR HPLC COA Datasheet MSDS
Purity 98.00%
HNMR COA Datasheet MSDS
Biological Description
Chemical Properties
Storage & Solubility Information
Description PD1-PDL1 inhibitor 2 is an inhibitor of the PD-1 (Programmed death- 1) /PD-Ll (Programmed death-ligand 1) protein/protein interaction, extracted from patent WO/2015034820 A1.
Targets&IC50 PD-1/PD-L1 :ic50 0.018μM,  
In vitro BMS-202 inhibits PD-1/PD-Ll interaction, and may augment therapeutic immune response to a number of histologically distinct tumors. Blockade of the PD-1/PD-Ll ligation using antibodies to PD-Ll has been shown to restore and augment T cell activation in many systems[1].
Kinase Assay All binding studies are performed in an HTRF assay buffer consisting of dPBS supplemented with 0.1% (with v) bovine serum albumin and 0.05% (v/v) Tween-20. For the PD-l-Ig/PD-Ll-His binding assay, inhibitors are pre-incubated with PD-Ll-His (10 nM final) for 15 m in 4 μL of assay buffer, followed by addition of PD-l-Ig (20 nM final) in 1 μL of assay buffer and further incubation for 15 m. PD-L1 from either human, cyno, or mouse are used. HTRF detection is achieved using europium crypate-labeled anti- Ig (1 nM final) and allophycocyanin (APC) labeled anti-His (20 nM final). Antibodies are diluted in HTRF detection buffer and 5 μL is dispensed on top of binding reaction. The reaction mixture is allowed to equilibrate for 30 minutes and signal (665 nm/620 nm ratio) is obtained using an En Vision fluorometer. Additional binding assays are established between PD-1-Ig/PD-L2-His (20, 5 nM, respectively), CD80-His/PD-Ll-Ig (100, 10 nM, respectively) and CD80-His/CTLA4-Ig (10, 5 nM, respectively).

Description

PD1-PDL1 inhibitor 2 is an inhibitor of the PD-1 (Programmed death- 1) /PD-Ll (Programmed death-ligand 1) protein/protein interaction, extracted from patent WO/2015034820 A1.

Targets&IC50

PD-1/PD-L1 :ic50 0.018μM

In Vitro

BMS-202 inhibits PD-1/PD-Ll interaction, and may augment therapeutic immune response to a number of histologically distinct tumors. Blockade of the PD-1/PD-Ll ligation using antibodies to PD-Ll has been shown to restore and augment T cell activation in many systems[1].

Kinase Assay

All binding studies are performed in an HTRF assay buffer consisting of dPBS supplemented with 0.1% (with v) bovine serum albumin and 0.05% (v/v) Tween-20. For the PD-l-Ig/PD-Ll-His binding assay, inhibitors are pre-incubated with PD-Ll-His (10 nM final) for 15 m in 4 μL of assay buffer, followed by addition of PD-l-Ig (20 nM final) in 1 μL of assay buffer and further incubation for 15 m. PD-L1 from either human, cyno, or mouse are used. HTRF detection is achieved using europium crypate-labeled anti- Ig (1 nM final) and allophycocyanin (APC) labeled anti-His (20 nM final). Antibodies are diluted in HTRF detection buffer and 5 μL is dispensed on top of binding reaction. The reaction mixture is allowed to equilibrate for 30 minutes and signal (665 nm/620 nm ratio) is obtained using an En Vision fluorometer. Additional binding assays are established between PD-1-Ig/PD-L2-His (20, 5 nM, respectively), CD80-His/PD-Ll-Ig (100, 10 nM, respectively) and CD80-His/CTLA4-Ig (10, 5 nM, respectively).

Synonyms BMS-202, PD-1/PD-L1 inhibitor 2
Purity 98.00%
Appearance solid
Molecular Weight 419.52
Formula C25H29N3O3
CAS No. 1675203-84-5

Storage

-20℃ 3 years powder

-80℃ 2 years in solvent

Solubility Information

DMSO: 76 mg/mL (181.2 mM)

Ethanol: 83 mg/mL (197.85 mM)

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Louis S. Chupak, et al. Compounds useful as immunomodulators. WO 2015034820 A1

Related Compound Libraries

This product is contained In the following compound libraries：
Bioactive Compound Library Inhibitor Library Anti-cancer Compound Library Apoptosis Compound Library Immunology/Inflammation Compound Library Cell cycle related Compound Library

Related Products

Related compounds with same targets
BMS202 hydrochloride (1675203-84-5(free base)) Phellodendrine chloride PD-1/PD-L1 inhibitor 1 Avelumab (anti- PD-L1) Atezolizumab (anti-PD-L1) BMS-1001 PD1-PDL1 inhibitor 2 Nivolumab (anti-PD-1)

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