Powder: -20°C for 3 years | In solvent: -80°C for 1 year
PF-431396 is a dual PYK2/FAK inhibitor (IC50: 11/2 nM).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
5 mg | In stock | $ 50.00 | |
10 mg | In stock | $ 74.00 | |
25 mg | In stock | $ 148.00 | |
50 mg | In stock | $ 261.00 | |
100 mg | In stock | $ 447.00 | |
200 mg | In stock | $ 659.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 59.00 |
Description | PF-431396 is a dual PYK2/FAK inhibitor (IC50: 11/2 nM). |
Targets&IC50 | FAK:2 nM, PYK2:11 nM |
In vivo | PF-431396 consistently inhibits protein tyrosine kinase phosphorylation under both calcium deficiency and the presence of Ca/W-7. It also blocks tyrosine phosphorylation of Pyk2 and FAK induced by anti-Ig- and aggregated LFA-1 in A20 cells, thereby inhibiting B cell proliferation. |
Kinase Assay | In Vitro Kinase Assays and Selectivity Screening : To measure Mps1 activity, 25 ng of recombinant, full-length enzyme is incubated in reaction buffer (50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 0.01% Triton X-100 and 5 μM Myelin basic protein (MBP)) containing vehicle (DMSO alone) or inhibitors. Forty μM ATP (2xKm) is added with 1 μCi [γ-33P]ATP and the reaction is incubated at room temperature for 45 minutes. Reactions are terminated with 3% phosphoric acid and transferred to P81 filter plates. Samples are washed in 1% phosphoric acid and 33P radioactivity is measured on a TopCount scintillation reader. In-house kinase assays are all carried out at 2xKm ATP concentrations. MPI-0479605 (500 nM) is also screened against a larger kinase panel. |
Molecular Weight | 506.5 |
Formula | C22H21F3N6O3S |
CAS No. | 717906-29-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 25.3 mg/mL (50 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
PF-431396 717906-29-1 Angiogenesis Cytoskeletal Signaling Tyrosine Kinase/Adaptors FAK PYK2 inhibit Inhibitor Proline-rich tyrosine kinase 2 PF 431396 Pyk2 PTK2 PF431396 Focal adhesion kinase PTK2 protein tyrosine kinase 2 inhibitor