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PJ34 hydrochloride

Catalog No. T2124   CAS 344458-15-7
Synonyms: PJ34 HCl

PJ34 hydrochloride (PJ34 HCl) is a potent specific inhibitor of PARPl/2.

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PJ34 hydrochloride Chemical Structure
PJ34 hydrochloride, CAS 344458-15-7
Pack Size Availability Price/USD Quantity
10 mg In stock $ 48.00
25 mg In stock $ 89.00
50 mg In stock $ 148.00
100 mg In stock $ 239.00
200 mg In stock $ 359.00
1 mL * 10 mM (in DMSO) In stock $ 83.00
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Purity: 98.87%
Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description PJ34 hydrochloride (PJ34 HCl) is a potent specific inhibitor of PARPl/2.
Targets&IC50 PARP:20 nM(EC50)
Kinase Assay To assess the PARP-1 or PARP-2 inhibitory activity of FR247304, 3-AB, and PJ34, PARP activity is evaluated with minor modifications. PARP enzyme assay is carried out in a final volume of 100 μL consisting of 50 mM Tris-HCl (pH 8.0), 25 mM MgCl2, 1 mM dithiothreitol, 10 μg activated salmon sperm DNA, 0.1 μCi of [adenylate-32P]NAD, 0.2 units of recombinant human PARP for PARP-1 assay or 0.1 units of recombinant mouse PARP-2 for PARP-2 assay, and various concentrations of FR261529 or 3-AB. The reaction mixture is incubated at room temperature (23°C) for 15 min, and the reaction is terminated by adding 200 μL of ice-cold 20% trichloroacetic acid (TCA) and incubated at 4°C for 10 min. The precipitate is transferred onto GF/B filter and washed three times with 10% TCA solution and 70% ethanol. After the filter is dried, the radioactivity is determined by liquid scintillation counting.
Cell Research PJ34 is dissolved in 100% DMSO at 10 mM and then diluted in DMEM without serum[1]. PC12 cell cultured are grown in Dulbecco's modified Eagle's medium supplemented with 5% (v/v) fetal calf serum, 5% (v/v) horse serum, and a 1% (v/v) penicillin-streptomycin antibiotics mixture. Cells are grown in an atmosphere of 95% air and 5% CO2 at 37°C. For all experiment, cells are seeded at a density of 4×104 cells/well in 96-well culture plates and allowed to attach overnight. For assessment of cell viability, hydrogen peroxide-induced cytotoxicity is quantified by a standard measurement of LDH release with the use of the LDH assay kit. Briefly, 6 h after hydrogen peroxide exposure, 20 μL of medium of each well is collected, and the solution prepared from LDH assay kit is added. After incubation at room temperature for 30 min, the reaction is stopped by addition of 1 N HCl, and absorbance is measured at 450 nm using a microplate reader.
Synonyms PJ34 HCl
Molecular Weight 331.8
Formula C17H18ClN3O2
CAS No. 344458-15-7

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 33.2 mg/mL (100 mM)

H2O: 33.2 mg/mL (100 mM)

TargetMolReferences and Literature

1. Garcia Soriano F, et al. Nat Med, 2001, 7(1), 108-113. 2. Scott GS, et al. J Pharmacol Exp Ther, 2004, 310(3), 1053-1061. 3. Mabley JG, et al. Inflamm Res, 2001, 50(11), 561-569.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Prostate Cancer Compound Library Anti-Pancreatic Cancer Compound Library NO PAINS Compound Library Bioactive Compound Library Anti-Ovarian Cancer Compound Library Immunology/Inflammation Compound Library Anti-Cancer Compound Library Bioactive Compounds Library Max Anti-Aging Compound Library Epigenetics Compound Library

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Keywords

PJ34 hydrochloride 344458-15-7 Chromatin/Epigenetic DNA Damage/DNA Repair PARP poly ADP ribose polymerase PJ 34 PJ 34 Hydrochloride inhibit PJ-34 Hydrochloride PJ34 PJ34 HCl PJ-34 PJ34 Hydrochloride PJ-34 hydrochloride Inhibitor inhibitor

 

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