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Pregnenolone

Catalog No. T0851 CAS 145-13-1

Synonyms: 3β-Hydroxy-5-pregnen-20-one, Arthenolone

Pregnenolone is an endogenous steroid hormone synthesized from cholesterol, used in the treatment of Alzheimer's disease.

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Pregnenolone, CAS 145-13-1

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Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Pregnenolone is an endogenous steroid hormone synthesized from cholesterol, used in the treatment of Alzheimer's disease.
In vitro	Pregnenolone induces a large, dose-related increase of both the rate and extent of MAP2-induced tubulin assembly, whereas progesterone, inactive per se, counteracted the stimulatory effect of Pregnenolone. Pregnenolone-increased assembly of microtubules produces a completely normal structure. [1] Pregnenolone preserves microtubule abundance and promotes cell movement during epiboly. [2] Pregnenolone results in dramatic reduction in GR nuclear localization in mouse hippocampal cell line (HT-22). Pregnenolone has neuroprotective effects against both glutamate and amyloid beta protein neuropathology. [3]
In vivo	Pregnenolone sustains its proliferative activity in vivo and stimulates the growth of LNCaP-tumor xenografts in intact male SCID mice as well as in castrated animals. Pregnenolone is shown to activate transcription through the LNCaP androgen receptor (AR), but not the wild-type AR. [4] Pregnenolone results in a significant decrease in the accumulation of astrocytes in the proximity of the wound and in a decreased bromodeoxyuridine incorporation in reactive astrocytes of rats. [5] Pregnenolone administration results in elevations in downstream neurosteroids such as allopregnanolone, a molecule with neuroprotective effects that also increases neurogenesis, decreases apoptosis and inflammation, modulates the hypothalamic-pituitary-adrenal axis, and markedly increases GABA(A) receptor responses. Pregnenolone administration elevates pregnenolone sulfate, a neurosteroid that positively modulates NMDA receptors. [6]

Synonyms	3β-Hydroxy-5-pregnen-20-one, Arthenolone
Molecular Weight	316.4776
Formula	C21H32O2
CAS No.	145-13-1

Storage

Powder: -20°C for 3 years

In solvent: -80°C for 2 years

Solubility Information

Ethanol: 21 mg/mL (66.4 mM)

DMSO: 21 mg/mL (66.4 mM)

H2O: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

References and Literature

1. Murakami K, et al. Proc Natl Acad Sci U S A, 2000, 97(7), 3579-3584. 2. Hsu HJ, et al. Nature,2006, 439(7075), 480-483. 3. Gursoy E, et al. Neurochem Res,2001, 26(1), 15-21. 4. Grigoryev DN, et al. J Steroid Biochem Mol Biol, 2000, 75(1), 1-10. 5. García-Estrada J, et al. Int J Dev Neurosci. 1999 Apr;17(2):145-51.

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Pregnenolone 145-13-1 Autophagy GPCR/G Protein Membrane transporter/Ion channel Metabolism Neuroscience Cannabinoid Receptor Endogenous Metabolite AChR TRP/TRPV Channel 3β-Hydroxy-5-pregnen-20-one Transient receptor potential channels inhibit TRP Channel Inhibitor Arthenolone inhibitor

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