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RI-1

Catalog No. T2276   CAS 415713-60-9
Synonyms: RI1, RAD51 inhibitor 1

RI-1 (RAD51 inhibitor 1) is a RAD51 inhibitor (IC50: 5-30 μM).

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RI-1 Chemical Structure
RI-1, CAS 415713-60-9
Pack Size Availability Price/USD Quantity
2 mg In stock $ 37.00
5 mg In stock $ 59.00
10 mg In stock $ 105.00
25 mg In stock $ 162.00
50 mg In stock $ 198.00
100 mg In stock $ 369.00
1 mL * 10 mM (in DMSO) In stock $ 66.00
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Purity: 99.92%
Purity: 99.62%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description RI-1 (RAD51 inhibitor 1) is a RAD51 inhibitor (IC50: 5-30 μM).
Targets&IC50 RAD51:<30 μM
Kinase Assay DNA binding assays: All reactions are performed in black non-binding polystyrene 384-well plates with reaction volumes of 30–100 μL. Purified DNA strand exchange proteins and chemical compounds are pre-incubated at room temperature for 5 minutes; they are then further incubated at 37°C for 30 min with 100 nM of ssDNA substrate, consisting of a 45-mer poly-dT tagged with Alexa 488 at the 5' terminus (synthesized and purified by Integrated DNA Technologies). Reactions are performed in 20 mM HEPES pH 7.5, 10 mM MgCl2, 0.25 μM BSA, 2% glycerol, 30 mM NaCl, 4% DMSO and 2 mM ATP. Some conditions included DTT or TCEP (tris(2-carboxyethyl)phosphine) as indicated. DNA binding is measured as a function of fluorescence polarization (FP) with a Safire2 plate reader, using the following settings: excitation 470±5 nM, emission 530±5 nM, 10 reads/well, Z height and G factor auto-calibrated from control wells. Displayed error bars represent standard deviation. For experiments involving a titration of protein concentrations, data are fit to an equation that accounts for the cooperative nature by which recombinase proteins bind DNA. For experiments involving a titration of RI-1, protein concentrations are selected to give an ~80% saturation of the FP signal in the absence of RI-1.
Cell Research Cytotoxicity is determined by loss of colony-forming ability. Experiments are performed in triplicate. Crystal violet stained colonies are imaged with a CCD camera and counted using NIH Image software. Error bars denote standard error.(Only for Reference)
Synonyms RI1, RAD51 inhibitor 1
Molecular Weight 361.61
Formula C14H11Cl3N2O3
CAS No. 415713-60-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 36.2 mg/mL (100 mM)

TargetMolReferences and Literature

1. Budke B, et al. Nucleic Acids Res. 2012, 40(15), 7347-7357. 2. Bee L, et al. PLoS One. 2013, 8(7), e69061.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Aging Compound Library Bioactive Compounds Library Max NO PAINS Compound Library Anti-Cancer Compound Library Inhibitor Library Cell Cycle Compound Library Bioactive Compound Library DNA Damage & Repair Compound Library Toxic Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

RI-1 415713-60-9 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis recombination RI1 homologous RI 1 Inhibitor RAD51 RAD51 inhibitor 1 inhibit inhibitor

 

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