Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RI-1 (RAD51 inhibitor 1) is a RAD51 inhibitor (IC50: 5-30 μM).
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
2 mg | In stock | $ 37.00 | |
5 mg | In stock | $ 59.00 | |
10 mg | In stock | $ 105.00 | |
25 mg | In stock | $ 162.00 | |
50 mg | In stock | $ 198.00 | |
100 mg | In stock | $ 369.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 66.00 |
Description | RI-1 (RAD51 inhibitor 1) is a RAD51 inhibitor (IC50: 5-30 μM). |
Targets&IC50 | RAD51:<30 μM |
Kinase Assay | DNA binding assays: All reactions are performed in black non-binding polystyrene 384-well plates with reaction volumes of 30–100 μL. Purified DNA strand exchange proteins and chemical compounds are pre-incubated at room temperature for 5 minutes; they are then further incubated at 37°C for 30 min with 100 nM of ssDNA substrate, consisting of a 45-mer poly-dT tagged with Alexa 488 at the 5' terminus (synthesized and purified by Integrated DNA Technologies). Reactions are performed in 20 mM HEPES pH 7.5, 10 mM MgCl2, 0.25 μM BSA, 2% glycerol, 30 mM NaCl, 4% DMSO and 2 mM ATP. Some conditions included DTT or TCEP (tris(2-carboxyethyl)phosphine) as indicated. DNA binding is measured as a function of fluorescence polarization (FP) with a Safire2 plate reader, using the following settings: excitation 470±5 nM, emission 530±5 nM, 10 reads/well, Z height and G factor auto-calibrated from control wells. Displayed error bars represent standard deviation. For experiments involving a titration of protein concentrations, data are fit to an equation that accounts for the cooperative nature by which recombinase proteins bind DNA. For experiments involving a titration of RI-1, protein concentrations are selected to give an ~80% saturation of the FP signal in the absence of RI-1. |
Cell Research | Cytotoxicity is determined by loss of colony-forming ability. Experiments are performed in triplicate. Crystal violet stained colonies are imaged with a CCD camera and counted using NIH Image software. Error bars denote standard error.(Only for Reference) |
Synonyms | RI1, RAD51 inhibitor 1 |
Molecular Weight | 361.61 |
Formula | C14H11Cl3N2O3 |
CAS No. | 415713-60-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 36.2 mg/mL (100 mM)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
RI-1 415713-60-9 Cell Cycle/Checkpoint DNA Damage/DNA Repair DNA/RNA Synthesis recombination RI1 homologous RI 1 Inhibitor RAD51 RAD51 inhibitor 1 inhibit inhibitor