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RU.521

Catalog No. T5486 CAS 2262452-06-0

Synonyms: RU320521

RU.521 (RU3205217) is a potent dsDNA inhibitor (IC50 : 700 nM), inhibition of the dsDNA pathway by RU.521 requires the presence of cGAS.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

RU.521, CAS 2262452-06-0

Pack Size	Availability	Price/USD
1 mg	In stock	$ 41.00
2 mg	In stock	$ 58.00
5 mg	In stock	$ 97.00
10 mg	In stock	$ 172.00
25 mg	In stock	$ 281.00
50 mg	In stock	$ 396.00
100 mg	In stock	$ 589.00
1 mL * 10 mM (in DMSO)	In stock	$ 97.00

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Purity: 99.82%

Purity: 98%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	RU.521 (RU3205217) is a potent dsDNA inhibitor (IC50 : 700 nM), inhibition of the dsDNA pathway by RU.521 requires the presence of cGAS.
Targets&IC50	dsDNA:700 nM
In vivo	Treatment of primary BMDMs with RU.521 or its analogs reduced IFNB1 expression in mice, indicating their effectiveness in suppressing intrinsic DNA-dependent, constitutively-activated type I interferon expression in cells deficient of a cytoplasmic DNA exonuclease.
Cell Research	Small-molecule compounds were serially diluted to concentrations spanning the range tested in the response curves were added to 6.7?×?10^5 RAW-Blue macrophages plated 16?h prior in 96-well dishes, then harvested 72?h after compound addition. ATP was measured using CellTiter Glo Viability Assay using 50?μM Tamoxifen as a positive control for cytotoxicity. Viability values were generated using vehicle (DMSO) or the first dose as 100% and Tamoxifen as 0%. Outliers were removed.
Animal Research	the chronically elevated levels of cytokines observed in Trex1 null mice are a consequence of constitutively activated cGAS, due to the inability to eliminate aberrantly localized self-DNA. We harvested BMDMs from 6–8-week old Trex1 ?/? mice, treated them with each compound, and measured expression levels of IFNB1 by quantitative reverse transcription PCR (qRT-PCR). Treatment of primary BMDMs with RU.521 or its analogs reduced IFNB1 expression, indicating their effectiveness in suppressing intrinsic DNA-dependent, constitutively-activated type I interferon expression in cells deficient of a cytoplasmic DNA exonuclease.

Synonyms	RU320521
Molecular Weight	415.23
Formula	C19H12Cl2N4O3
CAS No.	2262452-06-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 125 mg/mL (301.04 mM)

References and Literature

1. Vincent J, et al. Small molecule inhibition of cGAS reduces interferon expression in primary macrophages from autoimmune mice. Nat Commun. 2017 Sep 29;8(1):750.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compounds Library Max NO PAINS Compound Library Immunology/Inflammation Compound Library Bioactive Compound Library DNA Damage & Repair Compound Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

RU.521 2262452-06-0 DNA Damage/DNA Repair Immunology/Inflammation DNA cGAS inflammatory oxidative stress Inhibitor inhibit RU 320521 Cyclic GMP-AMP Synthase RU-320521 RU320521 signaling pathway inhibitor

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