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Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SYBR Green I is an asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.
Pack Size | Availability | Price/USD | Quantity |
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100 μL | Inquiry | $ 218.00 |
Description | SYBR Green I is an asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm. |
In vitro | Precasting SYBR Green I Gels:Precast nondenaturing polyacrylamide or agarose gels with SYBR Green I staining solution, which is diluted from the SYBR Green I stock reagent 1:10,000 prior to pouring the gel. The DNA detection limit for gels precast with SYBR Green I stain may be slightly higher (on the order of 30 to 40 pg/band) than for gels stained after electrophoresis (less than 20 pg/band), and the migration rate of DNA fragments in SYBR Green I gels may be significantly slower than the migration rate of the same fragments in a gel containing no dye. |
Synonyms | SYBR(R) GREEN I NUCLEIC ACID GEL STAIN *10,000× concentrate in DMSO |
Molecular Weight | 509.74 |
Formula | C32H37N4S |
CAS No. | 163795-75-3 |
keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
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SYBR GREEN 1 163795-75-3 Others SYBR(R) GREEN I NUCLEIC ACID GEL STAIN *10,000× concentrate in DMSO inhibitor inhibit