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Selonsertib

Catalog No. T3350 CAS 1448428-04-3

Synonyms: GS-4997

Selonsertib (GS-4997) is an orally bioavailable inhibitor of apoptosis signal-regulating kinase 1 (ASK1), with potential anti-inflammatory, antineoplastic and anti-fibrotic activities.

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Selonsertib, CAS 1448428-04-3

Pack Size	Availability	Price/USD
2 mg	In stock	$ 45.00
5 mg	In stock	$ 72.00
10 mg	In stock	$ 93.00
25 mg	In stock	$ 159.00
50 mg	In stock	$ 202.00
100 mg	In stock	$ 363.00
200 mg	In stock	$ 522.00
500 mg	In stock	$ 847.00
1 mL * 10 mM (in DMSO)	In stock	$ 78.00

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Purity: 99.53%

Purity: 98.97%

Purity: 95.04%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Selonsertib (GS-4997) is an orally bioavailable inhibitor of apoptosis signal-regulating kinase 1 (ASK1), with potential anti-inflammatory, antineoplastic and anti-fibrotic activities.
Targets&IC50	ASK1:8.3(pIC50)
Kinase Assay	Kinase assays mTOR.: Reagents are prepared as follows:"Simple Tor buffer": 10 mM Tris pH 7.4, 100 mM NaCl, 0.1% Tween-20, 1 mM DTT. Recombinant mTOR is diluted in this buffer to an assay concentration of 0.200ug/mL. ATP/Substrate solution: 0.075 mM ATP, 12.5 mM MnCl2, 50 mM Hepes, pH 7.4, 50 mM β-GOP, 250 nM Microcystin LR, 0.25 mM EDTA, 5 mM DTT, and 3.5 μg/mL GST-p70S6. Dilution Curve: A 10-point, 1:3 dilution of compounds are prepared in neat DMSO at 50 times the final assay concentration. Detection reagent mix: 50 mM HEPES, pH 7.4 0.01% Triton X-100, 0.01% BSA, 0.1 mM EDTA, 12.7 ug/mL Cy5-anti-GST antibody, 9 ng/ml anti-phospho p70S6 antibody (Thr389), 627ng/mL anti-mouse IgG labeled with Lance Eu. To 20 uL of the Simple Tor buffer is added 0.5 uL of the compound Dilution Curve in DMSO. The final concentration range for compound is 30 to 0.0015 μM. To initiate the reaction, 5 μL of the ATP/substrate solution is added to the above. The reaction is allowed to run for 60 minutes. The assay is stopped by adding 5 μL of 60 mM EDTA. Ten (10) μL of detection reagent mix is then added, and the mixture is allowed to sit at least 2 hours before reading on a Perkin Elmer Envision microplate reader set to detect Europium-based TR-FRET.

Synonyms	GS-4997
Molecular Weight	445.49
Formula	C24H24FN7O
CAS No.	1448428-04-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 34 mg/mL (76.3 mM)

Ethanol: 23 mg/mL (51.6 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. Lin JH, et al. Nephron. 2015, 129(1):29-33.

Citations

1. Yuan J, Yao C, Tang J, et al. Enhanced GRP78 protein expression via the IRE1α/ASK1/p38 MAPK pathway during As2O3-induced endoplasmic reticulum stress in BEAS-2B cells. Toxicology. 2021, 462: 152962.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Clinical Compound Library Drug Repurposing Compound Library Highly Selective Inhibitor Library Anti-Cancer Active Compound Library Anti-Cancer Drug Library Inhibitor Library Apoptosis Compound Library Anti-Metabolism Disease Compound Library Anti-Prostate Cancer Compound Library Pyroptosis Compound Library

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Selonsertib 1448428-04-3 Apoptosis MAPK ASK Inhibitor GS-4997 inhibit MAP3K GS4997 MAP kinase kinase kinase, MEKK, MAPKKK GS 4997 inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.