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Sonidegib

Catalog No. T1926   CAS 956697-53-3
Synonyms: Erismodegib, NVP-LDE225, LDE225

Sonidegib (Erismodegib), a Smoothened (Smo) antagonist, inhibits Hedgehog (Hh) signaling with IC50 of 1.3 nM (mouse) and 2.5 nM (human), respectively.

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Sonidegib Chemical Structure
Sonidegib, CAS 956697-53-3
Pack Size Availability Price/USD Quantity
5 mg In stock $ 54.00
10 mg In stock $ 76.00
25 mg In stock $ 118.00
50 mg In stock $ 164.00
100 mg In stock $ 274.00
1 mL * 10 mM (in DMSO) In stock $ 58.00
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Purity: 99.97%
Purity: 99.97%
Purity: 99.91%
Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Sonidegib (Erismodegib), a Smoothened (Smo) antagonist, inhibits Hedgehog (Hh) signaling with IC50 of 1.3 nM (mouse) and 2.5 nM (human), respectively.
Targets&IC50 Smo (mouse):1.3 nM, Smo (hμMan):2.5 nM
Cell Research LDE225 is prepared for assay by serial dilution in DMSO and then added to empty assay plates. TM3Hh12 cells (TM3 cells containing Hh-responsive reporter gene construct pTA-8xGli-Luc) are cultured in F12 Ham's/DMEM (1:1) containing 5% horse serum, 2.5% fetal bovine serum (FBS), and 15 mM HEPES, pH 7.3. Cells are harvested by trypsin treatment, resuspended in F12 Ham's/DMEM (1:1) containing 5% horse serum and 15 mM HEPES, pH 7.3, added to assay plates, and incubated with LDE225 for approximately 30 min at 37 °C in 5% CO2. Then 1 nM or 25 nM Ag1.5 is added to assay plates and incubated at 37 °C in the presence of 5% CO2. After 48 hours, either Bright-Glo or MTS reagent is added to the assay plates and luminescence or absorbance at 492 nm is determined. IC50 values, defined as the inflection point of the logistic curve, are determined by nonlinear regression of the Gli-driven luciferase luminescence or absorbance signal from MTS assay vs log10 (concentration) of LDE225 using the R statistical software pack (Only for Reference)
Synonyms Erismodegib, NVP-LDE225, LDE225
Molecular Weight 485.5
Formula C26H26F3N3O3
CAS No. 956697-53-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 90 mg/mL (185.4 mM)

Ethanol: 90 mg/mL (185.4 mM)

H2O: < 1 mg/mL (insoluble or slightly soluble)

TargetMolReferences and Literature

1. Pan SF, et al. ACS Med. Chem. Lett., 2010, 1 (3), 130–134. 2. Fendrich V, et al. Ann Surg, 2011, 254(5), 818-23. 3. Tauchi T, et al. Arthritis Res Ther, 2012, 14(Suppl 1), O43.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Clinical Compound Library Anti-Cancer Drug Library Anti-Cancer Approved Drug Library Anti-Cancer Active Compound Library Approved Drug Library Fluorochemical Library FDA-Approved & Pharmacopeia Drug Library Bioactive Compound Library Anti-Prostate Cancer Compound Library NO PAINS Compound Library

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Keywords

Sonidegib 956697-53-3 GPCR/G Protein Stem Cells Hedgehog/Smoothened Smo NVP-LDE-225 LDE-225 Erismodegib Inhibitor inhibit Smoothened LDE 225 NVP-LDE225 NVP-LDE 225 LDE225 inhibitor

 

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