Home Tools
Log in
Cart

Tenofovir Disoproxil

Catalog No. T7111   CAS 201341-05-1
Synonyms: GS 4331, Bis(POC)-PMPA

Tenofovir Disoproxil (GS 4331) is a nucleotide reverse transcriptase inhibitor to treat HIV and chronic Hepatitis B.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Tenofovir Disoproxil Chemical Structure
Tenofovir Disoproxil, CAS 201341-05-1
Pack Size Availability Price/USD Quantity
10 mg In stock $ 41.00
25 mg In stock $ 61.00
50 mg In stock $ 85.00
100 mg In stock $ 117.00
200 mg In stock $ 158.00
500 mg In stock $ 231.00
1 mL * 10 mM (in DMSO) In stock $ 45.00
Bulk Inquiry
Get quote
Select Batch  
Purity: 99.17%
Contact us for more batch information
Biological Description
Chemical Properties
Storage & Solubility Information
Description Tenofovir Disoproxil (GS 4331) is a nucleotide reverse transcriptase inhibitor to treat HIV and chronic Hepatitis B.
In vitro Human renal proximal tubular epithelial cell line (HK-2)cells were grown for 48 h followed by 24 to 72 h exposure to 0-28.8 μM TFV or vehicle, phosphate buffered saline (PBS). MTT (MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and Trypan blue indicated that TFV diminished cell viability at 24-72 h. TFV decreased ATP levels at 72 h when compared to vehicle, reflecting mitochondrial dysfunction. TFV increased the oxidative stress biomarkers of protein carbonylation and 4-hydroxynonenol (4-HNE) adduct formation. Tumor necrosis factor alpha (TNFα) was released into the media following exposure to 14.5 and 28.8 μM TFV. Caspase 3 and 9 cleavage was induced by TFV compared to vehicle at 72 h. HK-2 cells are a sensitive model for TFV cytotoxicity and suggest that mitochondrial stress and apoptosis occur in HK-2 cells treated with TFV[1].
Cell Research Human immortalized epithelial HK-2 cells were grown in a keratinocyte-free media with 50 μg/mL bovine pituitary extract and 5 ng/mL recombinant epithelial growth factor from Invitrogen. Cells were grown in a warm humidified incubator with constant settings of 37 °C and 5% CO2. HK-2 cells were plated into six-well tissue culture plates (750,000 cells/mL) and allowed to grow for 48 h. Media was replaced and cells were treated with a final concentration of 0, 1.5, 3.0, 4.75, 14.5, or 28.8 μM TFV for 24, 48, or 72 h. The vehicle was an equal volume of phosphate buffered saline (PBS). Abacavir was prepared in sterile water and cells were treated for 24 h with 0, 1.5, 3 or 6 μM of abacavir to evaluate renal sensitivity to an agent recognized to be less nephrotoxic. Following the treatment period, cells were collected with Trypsin-EDTA (0.25%) for sample analysis[1].
Synonyms GS 4331, Bis(POC)-PMPA
Molecular Weight 519.44
Formula C19H30N5O10P
CAS No. 201341-05-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 38 mg/mL (73.16 mM)

TargetMolReferences and Literature

1. Rachel M , Reagan S , Brooke P , et al. Establishment of HK-2 Cells as a Relevant Model to Study Tenofovir-Induced Cytotoxicity[J]. International Journal of Molecular Sciences, 2017, 18(3):531-. 2. Menne S , Cote P J , Korba B E , et al. Antiviral Effect of Oral Administration of Tenofovir Disoproxil Fumarate in Woodchucks with Chronic Woodchuck Hepatitis Virus Infection[J]. Antimicrobial Agents and Chemotherapy, 2005, 49(7):2720-2728. 3. Fical L. Vývoj UHPLC-MS/MS metody pro analýzu vybraných antivirotik v HILIC a RP módu[J]. 2020 4. Fical L, Khalikova M, Kočová Vlčková H, et al. Determination of Antiviral Drugs and Their Metabolites Using Micro-Solid Phase Extraction and UHPLC-MS/MS in Reversed-Phase and Hydrophilic Interaction Chromatography Modes[J]. Molecules. 2021, 26(8): 2123. 5. Thieulent C, Hue E, Sutton G, et al. Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: efficacy of decitabine and valganciclovir alone or in combination[J]. Antiviral Research. 2020: 104931

TargetMolCitations

1. Thieulent C, Hue E, Sutton G, et al. Identification of antiviral compounds against equid herpesvirus-1 using real-time cell assay screening: efficacy of decitabine and valganciclovir alone or in combination. Antiviral Research. 2020: 104931 2. Fical L, Khalikova M, Kočová Vlčková H, et al. Determination of Antiviral Drugs and Their Metabolites Using Micro-Solid Phase Extraction and UHPLC-MS/MS in Reversed-Phase and Hydrophilic Interaction Chromatography Modes. Molecules. 2021, 26(8): 2123.

Related compound libraries

This product is contained In the following compound libraries:
Immunology/Inflammation Compound Library Approved Drug Library Nucleotide Compound Library Bioactive Compound Library Clinical Compound Library Orally Active Compound Library Anti-Viral Compound Library FDA-Approved Drug Library Drug Repurposing Compound Library Inhibitor Library

Related Products

Related compounds with same targets
Sophoranol Bifendate IR415 Entecavir Periglaucine A Mulberrofuran G Doxorubicin hydrochloride Chamaechromone

TargetMolDose Conversion

You can also refer to dose conversion for different animals. More

TargetMol In vivo Formulation Calculator (Clear solution)

Step One: Enter information below
Dosage
mg/kg
Average weight of animals
g
Dosing volume per animal
ul
Number of animals
Step Two: Enter the in vivo formulation
% DMSO
%
% Tween 80
% ddH2O
Calculate Reset

TargetMolCalculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculation
Molecular Weight Calculator
=
X
X

Molarity Calculator allows you to calculate the

  • Mass of a compound required to prepare a solution of known volume and concentration
  • Volume of solution required to dissolve a compound of known mass to a desired concentration
  • Concentration of a solution resulting from a known mass of compound in a specific volume
See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

X
=
X

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

=
/

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Tenofovir Disoproxil 201341-05-1 Microbiology/Virology Proteases/Proteasome HIV Protease Reverse Transcriptase HBV Inhibitor GS4331 HIV Human immunodeficiency virus GS 4331 GS-4331 Bis(POC)-PMPA inhibit Hepatitis B virus inhibitor

 

TargetMol