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WYC-209

Catalog No. T5341   CAS 2131803-90-0
Synonyms: WYC209

WYC-209 inhibits proliferation of malignant murine melanoma tumor-repopulating cells (TRCs, IC50: 0.19 uM). It targets the retinoic acid receptor (RAR).

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
WYC-209 Chemical Structure
WYC-209, CAS 2131803-90-0
Pack Size Availability Price/USD Quantity
5 mg In stock $ 55.00
10 mg In stock $ 89.00
25 mg In stock $ 189.00
50 mg In stock $ 297.00
100 mg In stock $ 487.00
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Purity: 98.91%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description WYC-209 inhibits proliferation of malignant murine melanoma tumor-repopulating cells (TRCs, IC50: 0.19 uM). It targets the retinoic acid receptor (RAR).
Targets&IC50 TRCs:0.19 μM
In vitro WYC-209 inhibits proliferation of malignant murine melanoma tumor-repopulating cells (TRCs), with an IC50 of 0.19?μM in a dose-dependent manner. WYC-209 also inhibits proliferation of TRCs of human melanoma, lung cancer, ovarian cancer, and breast cancer in culture.
In vivo WYC-209 abrogates 87.5% of lung metastases of melanoma TRCs in immune-competent wild-type C57BL/6 mice at 0.22?mg?kg-1 without showing apparent toxicity.
Cell Research Cell proliferation rate was measured by using MTT colorimetric assay. Cells were seeded in 3D soft fibrin gel in 96-well microplates at a density to maintain control (untreated) cells in an exponential phase of growth during the entire experiment. Cells were incubated with various concentrations of compounds for each time point followed by incubation with 50?μL MTT reagent for 3?h at 37?°C. After incubation, 150?μL MTT solvent was added and absorbance was measured at 570?nm. All experiments were repeated at least three times. The percentage of viable cells was calculated and averaged for each well: percent growth?=?(OD-treated cells/OD control cells)?×?100, cell proliferation at each time point is normalized to base line cell survival at the time of initial compound treatment (day 0).
Animal Research Four- to six-week-old female and male C57BL/6 mice were used in mice experiment. Mice were randomized into different groups. In metastasis experiment, B16-F1 cell spheroids were selected from 3D 90-Pa fibrin gels and pipetted to single cells. These TRCs were harvested and the cell number was counted under microscopy. The cells were then suspended in PBS with appropriate cell density. Thirty thousand TRCs were intravenously injected into the tail vein of each wild-type C57BL/6 mouse. Five days later, inoculated mice were intravenous implanted with 0.022?mg/kg WYC-209, 0.22?mg/kg WYC-209, or 0.1% DMSO every two days. The mice were euthanized and examined for lung tumor formation at day 30. In treated TRCs injected in vivo experiments, B16-F1 cells were seeded into 3D 90-Pa fibrin gels and treated with 10?μM WYC-209 or 0.1% DMSO on day 3, then isolated treated TRCs colonies on day 5 and pipetted into single cell. 30,000 of TRCs were intravenously injected into the tail vein of each wild-type C57BL/6 mouse. No blinding was performed in the mice experiments.
Synonyms WYC209
Molecular Weight 368.45
Formula C20H20N2O3S
CAS No. 2131803-90-0

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 80 mg/mL (217.13 mM)

H2O: Insoluble

TargetMolReferences and Literature

1. Chen J, et al. Inhibition of cancer stem cell like cells by a synthetic retinoid. Nat Commun. 2018 Apr 11;9(1):1406.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Active Compound Library Reprogramming Compound Library Anti-Cancer Compound Library Metabolism Compound Library Apoptosis Compound Library Neuronal Differentiation Compound Library Autophagy Compound Library Anti-Metabolism Disease Compound Library Bioactive Compounds Library Max Lipid Metabolism Compound Library

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Keywords

WYC-209 2131803-90-0 Apoptosis Autophagy Metabolism Retinoid Receptor WYC209 WYC 209 Retinoid X receptors Inhibitor Retinoic acid receptors RAR/RXR inhibit inhibitor

 

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