Powder: -20°C for 3 years | In solvent: -80°C for 1 year
YM201636 (IC50=33 nM), a specific PIKfyve inhibitor, is less effective to p110α and insensitive to Fabl, which is yeast orthologue.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 40.00 | |
2 mg | In stock | $ 58.00 | |
5 mg | In stock | $ 97.00 | |
10 mg | In stock | $ 183.00 | |
25 mg | In stock | $ 378.00 | |
50 mg | In stock | $ 592.00 | |
100 mg | In stock | $ 845.00 | |
500 mg | In stock | $ 1,730.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 98.00 |
Description | YM201636 (IC50=33 nM), a specific PIKfyve inhibitor, is less effective to p110α and insensitive to Fabl, which is yeast orthologue. |
Targets&IC50 | PIKfyve:33 nM |
In vivo | In NIH3T3 cells treated with serum starvation, YM201636 (0.8 μM) significantly reduces the production of PtdIns(3,5)P2 by up to 80%. In 3T3-L1 adipocytes, YM-201636 (IC50=54 nM) inhibits the absorption of 2-deoxyglucose, and at a dose of 160 nM, it fully inhibits the absorption of 2-deoxyglucose. In MDCK cells, YM201636 disrupts the continuous recycling of the intercellular junction proteins Claudin-1 and Claudin-2, leading to their accumulation within the cells and delaying the formation of epithelial barriers. |
Kinase Assay | Following 3T3L1 adipocyte serum-starvation and insulin stimulation, cell lysates containing protease inhibitors are clarified and then subjected to immunoprecipitation with anti-PIKfyve antibodies. Washed beads are mixed with 100 μM PtdIns and preincubated for 15 min with YM-201636 (100 nM) or vehicle in the assay buffer (50 mM Tris-HCl, pH 7.5, 1 mM EGTA and 10 mM MgCl2). The kinase assay (50 μL final volume) is carried out for 15 min at 37 °C with 15 μM ATP and [γ-32P]ATP (30 μCi). Lipids are extracted, spotted on TLC glass plates (250 μm), resolved by a chloroform/methanol/water/ammonia solvent system and detected by autoradiography[2]. |
Cell Research | YM-201636 is dissolved in DMSO and diluted with DMEM and added to cells at a final concentration of 800 nM. Cells are treated with YM-201636 or a DMSO control for 2 h. For TER measurements cells are plated at confluency on Transwell permeable polyester filters (0.4 μM pore size) with surface area of 0.33 cm2. Media is changed ever 2-3 days and cells are grown for 7 days prior to TER measurements[4]. |
Molecular Weight | 467.48 |
Formula | C25H21N7O3 |
CAS No. | 371942-69-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 33 mg/mL (70.6 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
YM-201636 371942-69-7 Autophagy Microbiology/Virology PI3K/Akt/mTOR signaling Influenza Virus PI3K Inhibitor Fab1 inhibit PIKfyve Phosphoinositide 3-kinase YM201636 YM 201636 inhibitor