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Kits Phosphatase Inhibitor Cocktail I (100× ddH2O)

Phosphatase Inhibitor Cocktail I (100× ddH2O)

Catalog No. C0002
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It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.

The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.

Pack Size Availability Price/USD Quantity
1 mL 29.00
1 mL * 10 205.00
1 mL * 50 841.00
1 mL * 100 1236.00
Bulk Size: Please Quote for Discount

Composition

Ingredient CAS MW Concentration (100x) Target Type
Sodium Fluoride 7681-49-4 41.99 100 mM Acid Phosphatase Reversible
Sodium Orthovanadate 13721-39-6 183.91 100 mM Alkaline phosphatase, PTPs, ATPases Reversible
Sodium Molybdate 7631-95-0 205.92 115 mM Acid and phosphoprotein Phosphatase Irreversible
Sodium Tartrate 868-18-8 194.05 400mM Acid Phosphatase Reversible
Imidazole 288-32-4 68.08 200 mM Alkaline phosphatase Reversible

Package

Attribute Value
Form Liquid
Formulation In 1 ml ddH2O
Storage -20 ℃, Avoid free/thaw
Freeze Ok to freeze
Toxicity Irritant

Handling Instruction

  • Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
  • Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
  • Briefly vortex cocktails to help facilitate the dissolution.

Features

  • Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
  • Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
  • Cost-Effective —— Lower price with higher quality

Notice

  • Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
  • If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.

Please follow the instructions