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Kits Phosphatase Inhibitor Cocktail III (2 Tubes, 100x)

Phosphatase Inhibitor Cocktail III (2 Tubes, 100x)

Catalog No. C0004
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It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein.

The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.

Pack Size Availability Price/USD Quantity
1 mL I + 1 mL II 43.00
(1 mL I + 1 mL II) * 10 321.00
(1 mL I + 1 mL II) * 50 1263.00
(1 mL I + 1 mL II) * 100 1853.00
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Composition

Tube No. Ingredient CAS MW Concentration (100x) Target Type
I (ddH2O) 1mL Sodium Fluoride 7681-49-4 41.99 100 mM Acid Phosphatase Reversible
Sodium Orthovanadate 13721-39-6 183.91 100 mM Alkaline phosphatase, PTPs, ATPases Reversible
Sodium Molybdate 7631-95-0 205.92 115 mM Acid and phosphoprotein Phosphatase Irreversible
Sodium Tartrate 868-18-8 194.05 400mM Acid Phosphatase Reversible
Imidazole 288-32-4 68.08 200 mM Alkaline phosphatase Reversible
II (DMSO) 1mL (-)-p-Bromotetramisole oxalate 62284-79-1 373.22 2.5 mM Alkaline phosphatases Irreversible
Cantharidin 56-25-7 196.2 500 μM Ser/Thr phosphatases Reversible
Microcystin LR 101043-37-2 995.17 500 nM Acid and PP1 and PP2A Reversible

Package

Attribute Value
Form Liquid
Formulation Tube I In 1 ml ddH2O
Tube II In 1 ml DMSO
Storage -20 ℃, Avoid free/thaw
Freeze Ok to freeze
Toxicity Irritant

Handling Instruction

  • Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
  • Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
  • Briefly vortex cocktails to help facilitate the dissolution.
  • Note to add tube I first, mix well, then add tube II, mix again.

Features

  • Accurate Ingredients —— Clearance of components and concentrations to avoid experimental uncertainty
  • Comprehensive Protection—— Effectively inhibit various proteases to protect proteins from being degraded
  • Cost-Effective —— Lower price with higher quality

Notice

  • Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
  • If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.