Measured by its esterase activity. The specific activity is >10 pmoles/min/μg.

Carbonic Anhydrase 4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31 kDa and the accession number is Q64444.

Mouse

HEK293 Cells

RP23-167D6.1,carbonic anhydrase IV,AW456718,Car4,Ca4

The Mouse Car4 (NP_031633.1) without the C-terminal propeptide (Met 1-Ser 277) was expressed,with a C-terminal polyhistidine tag.

> 98 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3-dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.