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Vinblastine sulfate

Catalog No. T1668 CAS 143-67-9

Synonyms: NSC49842, Vincaleukoblastine sulfate salt

Vinblastine sulfate (Vincaleukoblastine sulfate salt) can inhibit the formation of microtubule, it also inhibits nAChR(IC50=8.9 uM).

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Vinblastine sulfate, CAS 143-67-9

Pack Size	Availability	Price/USD
5 mg	In stock	$ 50.00
10 mg	In stock	$ 54.00
25 mg	In stock	$ 64.00
50 mg	In stock	$ 84.00
100 mg	In stock	$ 97.00
1 mL * 10 mM (in DMSO)	In stock	$ 63.00

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Purity: 99.38%

Purity: 98.75%

Purity: 97.81%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Vinblastine sulfate (Vincaleukoblastine sulfate salt) can inhibit the formation of microtubule, it also inhibits nAChR(IC50=8.9 uM).
Targets&IC50	nAChR:8.9 μM
In vitro	The average terminal half-lives of Vinblastine is 14.3 h. When incubated in freshly isolated rat hepatocytes, VLB penetrates rapidly and intensely into the cells, probably through a passive diffusion mechanism followed by tight cellular binding[3]. Vinblastine inhibits the angiogenic response induced by adrenomedullin and is also positive for mitotic slippage, causing micronuclei in mononucleate cells with cytokinesis block[4]. vinblastine gives significant increase in micronucleated mononucleated cells at concentrations that produced approximately 50% cell death and cytostasis or less as calculated using RPD, RICC and RCC[2].
In vivo	Vinblastine is a widely used anticancer drug with undesired side effects [6]. A combination of VBL and RAP at very low doses against human HCC gets a satisfactory antiangiogenic effect in vivo[4]. The clinically relevant dose of vinblastine inhibits palmitoylation of tubulin in vivo in CEM cells (effect on depalmitoylation of tubulin)[5].
Kinase Assay	Cell based receptor autophosphorylation assays: Autophosphorylation of PDGFR family kinase assays are cell-based enzyme-linked immunosorbent (ELISA) assays using CHO cells expressing wild-type PDGFRβ, chimeric protein PDGFRβ/c-Kit, and PDGFRβ/Flt3 which contain the extracellular and transmembrane domains of PDGFRβ and the cytoplasmic domain of c-Kit, and Flt-3. Cells are grown to confluency in 96-well microtiter plates under standard tissue culture conditions, followed by serum starvation for 16 hours. Briefly, quiescent cells are incubated at 37 °C with increasing concentrations of Tandutinib for 30 minutes followed by the addition of 8 nM PDGF-BB for 10 minutes. Cells are lysed in 100 mM Tris, pH 7.5, 750 mM NaCl, 0.5% Triton X-100, 10 mM sodium pyrophosphate, 50 mM NaF, 10 μg/mL aprotinin, 10 μg/mL leupeptin, 1 mM phenylmethylsulfonyl fluoride, 1 mM sodium vanadate, and the lysate is cleared by centrifugation at 15,000 g for 5 minutes. Clarified lysates are transferred into a second microtiter plate in which the wells are previously coated with 500 ng/well of 1B5B11 anti-PDGFRβ mAb and then incubated for 2 hours at room temperature. After washing three times with binding buffer (0.3% gelatin, 25 mM HEPES, pH 7.5, 100 mM NaCl, 0.01% Tween 20), 250 ng/mL of rabbit polyclonal anti-phosphotyrosine antibody is added and plates are incubated at 37 °C for 60 minutes. Subsequently, each well is washed three times with binding buffer and incubated with 1 μg/mL of horseradish peroxidase-conjugated anti-rabbit antibody at 37 °C for 60 minutes. Wells are washed prior to adding 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), and the rate of substrate formation is monitored at 650 nm.
Cell Research	Six-well treatment plates are set up that contained 5 × 104 cells/mL in each well, suspended in 3 mL culture medium, and these are treated with vinblastine for 3 h followed by 21 h growth. (Only for Reference)
Source	Natural Products > Apocynaceae > Catharanthus

Synonyms	NSC49842, Vincaleukoblastine sulfate salt
Molecular Weight	909.06
Formula	C46H58N4O9·H2SO4
CAS No.	143-67-9

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 90.9 mg/mL(100 mM)

Ethanol: < 1 mg/mL (insoluble or slightly soluble)

References and Literature

1. McKay DB, et al. Proc Soc Exp Biol Med. 1993, 203(3):372-376. 2. George E. Johnson, et al. Mutat Res. 2010, 702(2):189-192. 3. Zhou XJ, et al. Eur J Drug Metab Pharmacokinet. 1990, 15(4):323-332. 4. Ribatti D, et al. Oncogene. 2003, 22(41):6458-6461. 5. Joan M. Caron, et al. Chemotherapy. 2007, 53:51-58.

Citations

1. Wang D, Wang Y, Di X, et al.Cortical tension drug screen links mitotic spindle integrity to Rho pathway.Current Biology.2023

Related compound libraries

This product is contained In the following compound libraries：

Membrane Protein-targeted Compound Library Anti-Cancer Approved Drug Library Anti-Cancer Drug Library Anti-Cancer Clinical Compound Library Traditional Chinese Medicine Monomer Library Drug Repurposing Compound Library Anti-Cancer Active Compound Library Microtubule-Targeted Compound Library Inhibitor Library Anti-Neurodegenerative Disease Compound Library

Related Products

Related compounds with same targets

Mebendazole 10-Deacetyltaxol Entasobulin KHS 101 Verubulin hydrochloride Estramustine TN-16 CCB02

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Keywords

Vinblastine sulfate 143-67-9 Autophagy Cytoskeletal Signaling Neuroscience Microtubule Associated AChR inhibit Vincaleukoblastine Vincaleukoblastine Sulfate Microtubule/Tubulin Inhibitor Vinblastine Sulfate NSC 49842 Vinblastine NSC-49842 NSC49842 Vincaleukoblastine sulfate salt inhibitor

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