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Anti-AGT Monoclonal Antibody-AF555
Catalog No. TMAZ-0159A5
Alias Serpin A8, Angiotensinogen
Anti-AGT Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting AGT. Anti-AGT Monoclonal Antibody-AF555 can be used in ELISA, FACS.
Anti-AGT Monoclonal Antibody-AF555
Anti-AGT Monoclonal Antibody-AF555
Catalog No. TMAZ-0159A5Alias Serpin A8, Angiotensinogen
Anti-AGT Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting AGT. Anti-AGT Monoclonal Antibody-AF555 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $570 | 12 days | |
| 50 μg | $945 | 12 days | |
| 100 μg | $1,415 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-AGT Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting AGT. Anti-AGT Monoclonal Antibody-AF555 can be used in ELISA, FACS. |
| Alias | Serpin A8, Angiotensinogen |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of AGT overexpressed 435F cells with TMAZ-0159A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of AGT protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis|Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone (PubMed: 10619573, PubMed: 17138938). Acts by binding to angiotensin receptors AGTR1 and AGTR2 (PubMed: 1567413). Also binds the DEAR/FBXW7-AS1 receptor (By similarity)|Stimulates aldosterone release|Is a ligand for the G-protein coupled receptor MAS1 (By similarity). Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets (By similarity) |
| Conjucates | AF555 |
| Others Formats | Biotin/APC/PE/AF488/AF647 |
| Antibody Types Available | 5 |
| Immunogen | AGT |
| Antigen Species | Human |
| Gene ID | 183 |
| Uniprot ID | |
| Biology Area | Drug Target |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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