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Mdivi-1

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Catalog No. T1907Cas No. 338967-87-6
Alias Mitochondrial division inhibitor 1

Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.

Mdivi-1

Mdivi-1

🥰Excellent
Purity: 98.79%
Catalog No. T1907Alias Mitochondrial division inhibitor 1Cas No. 338967-87-6
Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.
Pack SizePriceAvailabilityQuantity
5 mg37 €In Stock
10 mg48 €In Stock
25 mg87 €In Stock
50 mg147 €In Stock
100 mg227 €In Stock
200 mg337 €In Stock
500 mg558 €In Stock
1 mL x 10 mM (in DMSO)40 €In Stock
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Purity:98.79%
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Product Introduction

Bioactivity
Description
Mdivi-1 (Mitochondrial division inhibitor 1) is a mitochondrial division inhibitor that inhibits DRP1 and Dynamin I (IC50=1-10 μM). Mdivi-1 inhibits mitochondrial autophagy.
Targets&IC50
Dnm1 GTPase:1 μM-10 μM
In vitro
In the ganglion cell layer (GCL), Mdivi-1 exhibits strong immunoreactivity. Twelve hours following ischemic induction, Mdivi-1 significantly increases protein expression within the GCL. It notably reduces the expression of GFAP protein without altering Drp1 protein expression. In the normal murine retina, Mdivi-1 primarily localizes to the inner plexiform layer, ganglion cell layer, outer plexiform layer, and inner nuclear layer. Early in the development of the ischemic murine retina, there is a marked increase in the protein expression of Mdivi-1 and glial fibrillary acidic protein (GFAP). Mdivi-1 inhibits apoptosis in the ischemic retina and significantly increases the survival rate of retinal ganglion cells (RGC) two weeks post-ischemia.
In vivo
Mdivi-1 inhibits the ATPase activity and self-assembly of Dnm1 by inducing a conformational change (IC50<10 μM). It effectively suppresses C8-Bid and STS-induced mitochondrial outer membrane permeabilization (MOMP) in HeLa cells and extracellular mouse liver mitochondria. Mdivi-1 blocks the divisionof Dynamin-related GTPases, yeast Dnm1, and human Drp1, facilitating efficient and reversible mitochondrial fusion into a net-like structure. Intracellularly, it prevents apoptosis by inhibiting mitochondrial outer membrane permeabilization. Mdivi-1 represents a potential therapeutic class for treating stroke, myocardial infarction, and neurodegenerative diseases.
Kinase Assay
All GTPase assay reactions are started in a 200 μL volume, of which 150 μL is placed into the well of a 96-well plate. Depletion of NADH, as monitored by reading the A340 of the reaction, is measured every 20 s for a total of 40 min using a SpectraMAX 250 96-well plate reader. Spectrophotometric data are transferred to Excel and the measured steady state depletion of NADH over time is converted to protein activity.
Cell Research
Mdivi-1 is dissolved in DMSO. YPGlycerol plates are topped with 10 mL YPGlycerol containing 1% low melt agar and 75 μM mdivi-1, and cells are spotted 12 hours later using a 48 well pinning device. After pinning cells, plates are incubated at 24°C or 37°C and imaged using an Eagle Eye II imaging system.
AliasMitochondrial division inhibitor 1
Chemical Properties
Molecular Weight353.22
FormulaC15H10Cl2N2O2S
Cas No.338967-87-6
SmilesO=C1N(C(=S)NC=2C1=CC=CC2)C3=CC(OC)=C(Cl)C=C3Cl
Relative Density.1.57 g/cm3 (Predicted)
Storage & Solubility Information
Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 50 mg/mL (141.55 mM)
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.53 mg/mL (9.99 mM), In vivo: Suspension. Please add co-solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
10 mM0.2831 mL1.4155 mL2.8311 mL14.1555 mL
20 mM0.1416 mL0.7078 mL1.4155 mL7.0777 mL
50 mM0.0566 mL0.2831 mL0.5662 mL2.8311 mL
100 mM0.0283 mL0.1416 mL0.2831 mL1.4155 mL

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