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Anti-CD86 Monoclonal Antibody-AF647

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Catalog No. TMAZ-0050A6
Alias T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen

Anti-CD86 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting CD86. Anti-CD86 Monoclonal Antibody-AF647 can be used in ELISA, FACS.

Anti-CD86 Monoclonal Antibody-AF647

Anti-CD86 Monoclonal Antibody-AF647

😃Good
Catalog No. TMAZ-0050A6Alias T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen
Anti-CD86 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting CD86. Anti-CD86 Monoclonal Antibody-AF647 can be used in ELISA, FACS.
Pack SizePriceAvailabilityQuantity
25 μg$31512 days
50 μg$52012 days
100 μg$78012 days
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Product Introduction

Bioactivity
Description
Anti-CD86 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting CD86. Anti-CD86 Monoclonal Antibody-AF647 can be used in ELISA, FACS.
AliasT-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen
Ig Type
Rabbit monoclonal IgG
Reactivity
Human
Verified Activity
Flow cytometry analysis of CD86 overexpressed 293F cells with TMAZ-0050A6BI, which was counjucated with SA-PE (red). The WT 293F control is black line.
Application
ELISA, FACS
Recommended Dose
0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA
Antibody Type
Monoclonal
Host SpeciesRabbit
Subcellular LocalizationSingle-pass type I membrane
ConstructionThis antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of CD86 protein.
PurificationProtein A Affinity Purified
AppearanceLiquid
Formulation0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin.
Purity> 95% as determined by SDS-PAGE.
Research BackgroundReceptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed: 12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed: 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity)|Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation|(Microbial infection) Acts as a receptor for adenovirus subgroup B
Related Conjugates and Formulations
Conjucates
AF647
Others Formats
Biotin/APC/PE/AF488/AF555
Antibody Types Available
2
Antigen Details
Immunogen
CD86
Antigen Species
Human
Gene ID
942
Uniprot ID
Biology Area
Immunology Research
Chemical Properties
Molecular Weight150 kDa
Stability & Storage
Stability & StorageFor unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze.
TransportFor unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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