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Anti-CD86 Monoclonal Antibody-APC
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Catalog No. TMAZ-0050A
Alias T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen
Anti-CD86 Monoclonal Antibody-APC is a Biotin-conjugated rabbit antibody targeting CD86. Anti-CD86 Monoclonal Antibody-APC can be used in ELISA, FACS.
Anti-CD86 Monoclonal Antibody-APC
Anti-CD86 Monoclonal Antibody-APC
😃Good
Catalog No. TMAZ-0050AAlias T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen
Anti-CD86 Monoclonal Antibody-APC is a Biotin-conjugated rabbit antibody targeting CD86. Anti-CD86 Monoclonal Antibody-APC can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $315 | 12 days | |
| 50 μg | $520 | 12 days | |
| 100 μg | $780 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-CD86 Monoclonal Antibody-APC is a Biotin-conjugated rabbit antibody targeting CD86. Anti-CD86 Monoclonal Antibody-APC can be used in ELISA, FACS. |
| Alias | T-lymphocyte activation antigen CD86, FUN-1, CTLA-4 counter-receptor B7.2, BU63, B70, Activation B7-2 antigen |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of CD86 overexpressed 293F cells with TMAZ-0050ABI, which was counjucated with SA-PE (red). The WT 293F control is black line. |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of CD86 protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4 (PubMed: 12196291). May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation (PubMed: 7527824). Also involved in the regulation of B cells function, plays a role in regulating the level of IgG(1) produced. Upon CD40 engagement, activates NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation (By similarity)|Interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation|(Microbial infection) Acts as a receptor for adenovirus subgroup B |
| Conjucates | APC |
| Others Formats | Biotin/PE/AF488/AF555/AF647 |
| Antibody Types Available | 2 |
| Immunogen | CD86 |
| Antigen Species | Human |
| Gene ID | 942 |
| Uniprot ID | |
| Biology Area | Immunology Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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