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Anti-EFNB2 Monoclonal Antibody-AF647
Catalog No. TMAZ-0210A6
Alias HTK ligand, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5
Anti-EFNB2 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting EFNB2. Anti-EFNB2 Monoclonal Antibody-AF647 can be used in ELISA, FACS.
Anti-EFNB2 Monoclonal Antibody-AF647
Anti-EFNB2 Monoclonal Antibody-AF647
Catalog No. TMAZ-0210A6Alias HTK ligand, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5
Anti-EFNB2 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting EFNB2. Anti-EFNB2 Monoclonal Antibody-AF647 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $395 | 12 days | |
| 50 μg | $655 | 12 days | |
| 100 μg | $980 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-EFNB2 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting EFNB2. Anti-EFNB2 Monoclonal Antibody-AF647 can be used in ELISA, FACS. |
| Alias | HTK ligand, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5 |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of EFNB2 overexpressed 429F cells with TMAZ-0210A6, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of EFNB2 protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons|(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus |
| Conjucates | AF647 |
| Others Formats | Biotin/APC/PE/AF488/AF555 |
| Antibody Types Available | 1 |
| Immunogen | EFNB2 |
| Antigen Species | Human |
| Gene ID | 1948 |
| Uniprot ID | |
| Biology Area | Cytokines |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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