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Anti-IL13 Monoclonal Antibody-AF647
Catalog No. TMAZ-0022A6
Alias Interleukin-13
Anti-IL13 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting IL13. Anti-IL13 Monoclonal Antibody-AF647 can be used in ELISA, FACS, IF.
Anti-IL13 Monoclonal Antibody-AF647
Anti-IL13 Monoclonal Antibody-AF647
Catalog No. TMAZ-0022A6Alias Interleukin-13
Anti-IL13 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting IL13. Anti-IL13 Monoclonal Antibody-AF647 can be used in ELISA, FACS, IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $570 | 12 days | |
| 50 μg | $945 | 12 days | |
| 100 μg | $1,415 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-IL13 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting IL13. Anti-IL13 Monoclonal Antibody-AF647 can be used in ELISA, FACS, IF. |
| Alias | Interleukin-13 |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of IL13 overexpressed 382F cells with TMAZ-0022A6, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS, IF |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of IL13 protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Cytokine that plays important roles in allergic inflammation and immune response to parasite infection (PubMed: 8096327, PubMed: 8097324). Synergizes with IL2 in regulating interferon-gamma synthesis (PubMed: 8096327). Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (PubMed: 7903680, PubMed: 8759755). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (By similarity). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Functions also on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils (PubMed: 8639787). Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6 (PubMed: 9013879). Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (PubMed: 21622864) |
| Conjucates | AF647 |
| Others Formats | Biotin/APC/PE/AF488/AF555 |
| Antibody Types Available | 2 |
| Immunogen | IL13 |
| Antigen Species | Human |
| Gene ID | 3596 |
| Uniprot ID | |
| Biology Area | Immunology Research |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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