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Anti-LCAT Monoclonal Antibody-PE
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Catalog No. TMAZ-0161P
Alias Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase
Anti-LCAT Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody-PE can be used in ELISA, FACS.
Anti-LCAT Monoclonal Antibody-PE
Anti-LCAT Monoclonal Antibody-PE
😃Good
Catalog No. TMAZ-0161PAlias Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase
Anti-LCAT Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody-PE can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $570 | 12 days | |
| 50 μg | $945 | 12 days | |
| 100 μg | $1,415 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-LCAT Monoclonal Antibody-PE is a Biotin-conjugated rabbit antibody targeting LCAT. Anti-LCAT Monoclonal Antibody-PE can be used in ELISA, FACS. |
| Alias | Platelet-activating factor acetylhydrolase, Phospholipid-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase, Lecithin-cholesterol acyltransferase, 1-alkyl-2-acetylglycerophosphocholine esterase |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of LCAT overexpressed 431F cells with TMAZ-0161P, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Single-pass type I membrane |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of LCAT protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs) (PubMed: 10329423, PubMed: 19065001, PubMed: 26195816). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16: 0-18: 2 or 18: O-18: 2 phosphatidylcholines (PubMed: 8820107). Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms (PubMed: 10722751). Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF) (PubMed: 8016111). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF (PubMed: 8016111). Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters (PubMed: 24620755) |
| Conjucates | PE |
| Others Formats | Biotin/APC/AF488/AF555/AF647 |
| Antibody Types Available | 1 |
| Immunogen | LCAT |
| Antigen Species | Human |
| Gene ID | 3931 |
| Uniprot ID | |
| Biology Area | Drug Target |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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