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Anti-PODXL Monoclonal Antibody-AF555
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Catalog No. TMAZ-0170A5
Alias Podocalyxin-like protein 1, Podocalyxin, Gp200, GCTM-2 antigen
Anti-PODXL Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting PODXL. Anti-PODXL Monoclonal Antibody-AF555 can be used in ELISA, FACS.
Anti-PODXL Monoclonal Antibody-AF555
Anti-PODXL Monoclonal Antibody-AF555
😃Good
Catalog No. TMAZ-0170A5Alias Podocalyxin-like protein 1, Podocalyxin, Gp200, GCTM-2 antigen
Anti-PODXL Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting PODXL. Anti-PODXL Monoclonal Antibody-AF555 can be used in ELISA, FACS.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 25 μg | $250 | 12 days | |
| 50 μg | $415 | 12 days | |
| 100 μg | $620 | 12 days |
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Product Introduction
Bioactivity
Related Conjugates and Formulations
Antigen Details
Chemical Properties
| Description | Anti-PODXL Monoclonal Antibody-AF555 is a Biotin-conjugated rabbit antibody targeting PODXL. Anti-PODXL Monoclonal Antibody-AF555 can be used in ELISA, FACS. |
| Alias | Podocalyxin-like protein 1, Podocalyxin, Gp200, GCTM-2 antigen |
| Ig Type | Rabbit monoclonal IgG |
| Reactivity | Human |
| Verified Activity | Flow cytometry analysis of PODXL overexpressed 377F cells with TMAZ-0170A5, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| Application | ELISA, FACS |
| Recommended Dose | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| Antibody Type | Monoclonal |
| Host Species | Rabbit |
| Subcellular Localization | Secreted |
| Construction | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of PODXL protein. |
| Purification | Protein A Affinity Purified |
| Appearance | Liquid |
| Formulation | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| Purity | > 95% as determined by SDS-PAGE. |
| Research Background | Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells |
| Conjucates | AF555 |
| Others Formats | Biotin/APC/PE/AF488/AF647 |
| Antibody Types Available | 2 |
| Immunogen | PODXL |
| Antigen Species | Human |
| Gene ID | 5420 |
| Uniprot ID | |
| Biology Area | Drug Target |
| Molecular Weight | 150 kDa |
Stability & Storage
| Stability & Storage | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| Transport | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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